Naslov
Change in postanatal rat offspring diet influences perigonadal white adipose tissue morphology in a sex-specific manner

Autori
Fenrich, Matija ; Habjanović, Karlo ; Čekić, Nenad ; Šnajder, Darija ; Bijelić, Nikola ; Radić, Radivoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
DAMEO Abstract book / - Osijek : Medicinski fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2018, 13-13

Skup
DAMEO

Mjesto i datum
Osijek, Hrvatska, 24.04.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
adipose tissue, image cytometry, Sprague-Dawley rats, high-fat diet

Sažetak
Introduction: Our previously published results suggest that maternal diet during gestation might influence the subcutaneous and visceral white adipose tissue morphology in the offspring. Objective of this study was to further investigate the combined effects of maternal and offspring diet on perigonadal white adipose tissue morphology in rats. Materials and methods: Ten female Sprague Dawley rats, nine weeks old, were randomly divided in two groups, CD (control diet) and HFD (food rich in saturated fatty acids) during five weeks, and then mated with genetically similar males. Offspring rats from each group (CD and HFD) were further randomized into four groups, regarding their own and their mothers' diet: CD-CD, CD- HFD, HFD-CD and HFD-HFD. Harvested perigonadal (i.e. scrotal and periovarian) adipose tissue samples were fixed in 4% paraformaldehide, paraffin-embedded, cut in 6 μm slices and dyed with haematoxylin-eosin. Histomorphometry and adipocyte quantification was done in the Cell Profiler. Results: Preliminary results of perigonadal adipose tissue quantification in female rats show significant morphological differences between CD-CD and CD-HFD rats and between CD-CD and HFD-CD rats in terms of hypertrophy (p=0, 016 and p=0, 038, respectively). Median areas of adipocytes in CD-HFD and HFD-CD rats are 1918, 16 μm² and 1623, 46 μm², respectively, which indicates slight hypertrophy compared to median areas in CD-CD (1224, 59 μm²). In male rats, adipocytes of HFD-HFD rats have a significantly smaller surface area compared to CD-CD (p=0, 026), CD-HFD (p=0, 026) and HFD-CD (p=0, 026) adipocyte surface area. Adipocytes of HFD-HFD male rats have a median surface area 1146, 13 μm² compared to 2127, 47 μm² of CD-CD rats, 2007, 68 μm² of CD-HFD rats and 1936, 53 μm² of HFD-CD rats. Conclusion: It might be suggested that change in postnatal diet of female offspring leads to periovarian adipocyte hypertrophy. In contrast, male rats show no significant change in adipocyte surface area, unless the maternal and offspring diet is rich in saturated fats leading to hypotrophy of scrotal adipocytes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA