Pregled bibliografske jedinice broj: 994784
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox // 5th Central-Eastern European Congress on Cell-Free Nucleic Acids
Debrecen, Mađarska, 2018. str. 47-47 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 994784 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Editing the epigenome using a comprehensive CRISPR/Cas9-based molecular toolbox
Autori
Vojta, Aleksandar ; Josipović, Goran ; Tadić, Vanja ; Zoldoš, Vlatka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
5th Central-Eastern European Congress on Cell-Free Nucleic Acids
Mjesto i datum
Debrecen, Mađarska, 21.06.2018. - 22.06.2018
Vrsta sudjelovanja
Pozvano predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
CRISPR ; Cas9 ; epigenetics ; gene regulation
Sažetak
The advent of the CRISPR/Cas9 technology revolutionized the field of genome engineering by providing a flexible platform for targeting almost any genomic region. Soon, fusions of the targeting moiety Cas9 with various effector domains enabled the construction of a novel class of molecular tools useful for gene regulation and epigenetic modifications. We focused on targeted CpG methylation and demethylation of gene promoters, which is a major epigenetic mechanism controlling gene expression. First, we constructed a modular molecular toolbox for introducing targeted epigenetic modifications, which enabled easy deployment and application to biological problems. After the challenging step of creating active fusions between Cas9 and DNMT3A or TET1 catalytic domains for CpG methylation/demethylation and careful characterization of the new molecular tools, we approached biological problems in the field of epigenetic regulation of protein glycosylation with special emphasis on the role of IgG glycosylation in inflammatory diseases. We are using this platform to reveal the underlying epigenetics responsible for the inflammatory phenotype common to many diseases with the aim of discovering robust biomarkers and also to pave the way for personalized and precise epigenetic therapy in the future.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb