Naslov
Distribution and elimination of levamisole in eggs and tissues after oral administration to laying hens, determined by LC-MS/MS

Autori
Solomun Kolanović, Božica ; Bilandžić, Nina ; Kos, Blaženka ; Šušković, Jagoda ; Cvetnić, Luka ; Varenina, Ivana ; Božić Luburić, Đurđica ; Varga, Ines ; Pavliček, Damir ; Denžić Lugomer, Marija ; Cvetnić, Željko

Izvornik
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment (1944-0049) 36 (2019), 5; 729-739

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
levamisole ; laying hens ; eggs ; tissues ; withdrawal time ; LC-MS/MS

Sažetak
Levamisole was administered to laying hens, and concentrations in eggs and tissues (thigh muscle, breast muscle, liver and kidney) were determined by a newly developed liquid chromatography tandem mass spectrometry method, which allowed trace level quantification of levamisole. The adopted analytical method showed good sensitivity, repeatability and percentage of recovery from spiked matrices. Maximum concentrations of levamisole were found on the first day after the administration (531.1 μg/kg in liver, 164.3 μg/kg in egg yolk, 130.7 μg/kg in kidney, 78.0 μg/kg in breast muscle, 70.7 μg/kg in thigh muscle and 64.0 μg/kg in egg white), after which there is a decline. The compound was rapidly eliminated from eggs, with a half-life of 1.3 days. Elimination appeared to be slower in thigh muscle (3.5 days), breast muscle (3.4 days) and liver (3.3 days). According to this experiment, the levamisole withdrawal periods calculated for eggs, liver, kidney, breast muscle and thigh muscle in laying hens were 14.1, 6.1, >4.0, 14.5 and 13.0 days, respectively. The longest time for levamisole residues to be completely released from tissues was seen in liver samples (37.4 days), followed by thigh muscle, breast muscle and kidney. Elimination from eggs was fastest (16.4 days for levamisole residues to drop below the method quantification limit).

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA