Pregled bibliografske jedinice broj: 994739
Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells
Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells // XLV ESAO Congress, New organs for life
Madrid, Španjolska, 2018. (predavanje, međunarodna recenzija, neobjavljeni rad, znanstveni)
CROSBI ID: 994739 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells
Autori
Ressler, Antonia
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni
Skup
XLV ESAO Congress, New organs for life
Mjesto i datum
Madrid, Španjolska, 12.09.2018. - 15.09.2018
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
chitosan, hydroxyapatite, hydrogels, mesenchymal stem cells, differentiation, osteogenesis
Sažetak
The goal of this study was to evaluate the potential of a pH-responsive chitosan-hydroxyapatite hydrogel to be used as a threedimensional support for encapsulated mesenchymal stem cells (MSCs) osteogenic differentiation. In vitro enzymatic degradation of the hydrogel, during 28 days of incubation, in simulated physiological condiditons, was characterized by swelling measurements, molecular weight determination and SEM analysis of hydrogel microstructure. Osteogenic differentiation of encapsulated MSCs was confirmed by osteogenic Runx2, collagen type I and osteocalcin immunostaining and alkaline phosphatase quantification. The deposition of late osteogenic markers (calcium phosphates) detected by Alizarin red and von Kossa staining indicated an extracellular matrix mineralization.
Izvorni jezik
Engleski
