Naslov
Silver in nano and bulk form differently affects photosynthesis and photosynthesis-related proteins in leaves of tobacco plants.

Autori
Balen, Biljana ; Tkalec, Mirta ; Peharec Štefanić, Petra ; Biba, Renata ; Cvjetko, Petra ; Šabarić, Jasenka ; Šikić, Sandra.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 2nd International Congress on Food Safety and Quality “Food Life Cycle” / Archives of Industrial Hygiene and Toxicology, 69, Suppl. / Šostar, Zvonimir ; Šikić, Sandra ; Krivohlavek, Adela - Zagreb, 2018, 37-37

Skup
2. međunarodni kongres o sigurnosti i kvaliteti hrane = 2nd International Congress on Food Safety and Quality

Mjesto i datum
Opatija, Hrvatska, 13.11.2018. - 16.11.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
chlorophyll fluorescence ; chloroplast ultrastructure ; nanosilver ; photosynthetic pigments ; proteomics

Sažetak
Nanotechnology has many applications in all stages of production of agricultural products. Among the different types of available nanomaterials, the most frequently used are silver nanoparticles (AgNPs) due to silver antimicrobial properties. As plants have a significant role in accumulation and biodistribution of many environmentally released substances, they can be influenced by AgNPs, serving as a potential pathway for transport and bioaccumulation of AgNPs into food chains. In this study, we investigated the effects of silver nano- and bulk-form on photosynthesis and leaf proteome of tobacco. Adult plants were exposed to 100 μmol L-1 citrate-coated AgNPs and AgNO3 and after 7 days of Ag accumulation, chlorophyll fluorescence and photosynthetic pigments as well as changes in leaf ultrastructure, and protein expression were analysed. Both types of treatments resulted with a similarly increased Ag uptake. AgNPs significantly decreased photochemical quenching and increased the concentration of violaxantin. On the contrary, treatments with AgNO3 did not significantly influence fluorescence parameters but had a negative effect on the majority of photosynthetic pigments. Moreover, after exposure to AgNPs, chloroplasts were smaller compared to chloroplasts in the control cells and somewhat swollen and ruptured, while those found in leaf cells of AgNO3-treated plants were bigger than the control ones. Majority of the identified proteins with differential expression were photosynthesis-related and down-regulated, although almost half of the proteins exhibited different expression levels between AgNPs and AgNO3 exposure. The obtained results indicate that the AgNPs effects observed in tobacco leaves were not simply due to the release of Ag ions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA