Naslov
Interaction of HPV E6 oncoproteins with specific proteasomal subunits.
(Interaction of HPV E6 oncoproteins with specific proteasomal subunits)

Autori
Tomaić, Vjekoslav ; Ganti, Ketaki ; Pim, David ; Bauer, Christina ; Blattner, Christine ; Banks, Lawrence

Izvornik
Virology (0042-6822) 446 (2013), 1-2; 389-396

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
E6 ; E6AP ; Human Papillomaviruses ; Proteasome ; S5a

Sažetak
The Human Papillomavirus E6 oncoproteins have the capacity to target several of their cellular interacting partners for proteasome mediated degradation, and recent proteomic analyses suggest a close involvement of E6 with the cellular proteasome machinery. In this study we have performed an extensive analysis of the capacity of different E6 oncoproteins to interact with specific proteasome components. We demonstrate that multiple subunits of the proteasome can be bound by different HPV E6 oncoproteins. Furthermore, whilst most of these interactions appear independent of the E6AP ubiquitin ligase, the association of E6 with the major ubiquitin-accepting proteasome subunit, S5a, does require the presence of E6AP. One consequence of the interaction between E6/E6AP and S5a is enhanced ubiquitination of this proteasome subunit. These results suggest a complex interplay between E6 and the proteasome, only some aspects of which are dependent upon the E6AP ubiquitin ligase.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA