Naslov
Influence of the encapsulation method on biological effect of quercetin and L-ascorbic acid

Autori
Huđek, Ana ; Durgo, Ksenija ; Bertoša, Rea ; Komes, Draženka ; Belščak-Cvitanović, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Esib / 2017, European Summit of Industrial Biotechnology / - Graz : Acib GmbH, 2017, 171-171

Skup
The European Summit of Industrial Biotechnology, ESIB2017 /Croatian - Austrian Science Day

Mjesto i datum
Graz, Austrija, 14.11.2017. - 16.11.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
encapsulation, biologically active compounds, antioxidant, cytotoxicity, synergism

Sažetak
During recent years, evaluation of bioactive compounds with antioxidant properties enabled the optimization of their properties and formulation of new functional properties aiming at their use in prevention and treatment of different health issues. The major drawback of using polyphenolic antioxidants as one of the most extensively used bioactive compounds, is their low water solubility, resulting in low bioavailability after consumption, bitter and acidic taste that limits their use in food and oral medicines, susceptibility to oxidation and degradation, and interaction with other food components. In order to overcome these disadvantages and improve the functional properties of polyphenols and other bioactive compounds, micro- and nano-encapsulation techniques are applied. To optimize the delivery of polyphenolic antioxidants, their combination with other bioactive compounds, potentially resulting in antioxidant synergism, needs to be evaluated and the delivery system formulated that will allow the best release and activity. In this paper, encapsulation of quercetin, L-ascorbic acid and amino acids lysine and carnitine in low viscosity grade alginate (A) and ethyl cellulose (EC) as polymeric carrier materials was examined. The physical and chemical properties and antioxidant effectiveness of bioactive compounds in free and encapsulated form were determined by chemical methods and by application of biological cellular models, human colon cancer cell line, CaCo-2 and laryngeal cancer cell line, HEp-2. By formulating mixtures of quercetin and ascorbic acid in combination with aminoacids lysine and carnitine in water solutions, synergistic effect on the enhancement of antioxidant capacity is achieved. The formulated quaternary mixture of all bioactive compounds, containing 0.1 gL-1 of quercetin and 10 gL-1 of L-ascorbic acid, lysine and carnitine showed cytoprotective and antioxidant activity on Hep-2 cell line. By employing ionic gelation of alginate and phase separation of ethyl cellulose as the encapsulation approaches, spherical particles ranging from microparticles (about 500 - 600 μm for EC particles) to macroparticles (<2 mm for A particles) were formulated, with the quaternary mixture of all four bioactive compounds. Alginate macroparticles in comparison to ethycellulose microparticles showed a significantly higher encapsulation efficiency of all bioactive compounds of the formulated mixture. Both A and EC particles encapsulating the bioactive mixture exhibited a highly prooxidative activity on CaCo-2 cell lines, among which alginate macroparticles with the encapsulated mixture had the most pronounced concentration-dependent effect. CaCo-2 cell line expressed less sensitivity to the activity of pure bioactive compounds and solution with particles with encapsulated bioactive mixture of compounds in relation to Hep-2 cell line.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Interdisciplinarne biotehničke znanosti

POVEZANOST RADA