Naslov
Size-exclusion high-performance liquid chromatography and sodium dodecyl sulfate- polyacrylamide gel electrophoresis of proteins: A comparison

Autori
Josić, Dj. ; Baumann, H. ; Reutter, W.

Izvornik
Analytical Biochemistry (0003-2697) 142 (1984), 2; 473-479

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, stručni

Ključne riječi
SE-HPLC of hydrophobic and hydrophilic proteins ; Influence of detergents ; Comparison between separation by SE-HPLC and SDS-PAGE

Sažetak
When size-exclusion HPLC (SE-HPLC) is applied for the separation of hydrophilic and hydrophobic proteins, numerous problems can be encountered, which may present considerable difficulties. A major source of such complications is interaction between column packing and sample, especially on the so-called “Diol” and “Polyol” columns. In many cases interaction can be reduced only by adding detergents. Calibration proteins and hydrophobic membrane proteins of liver are separated by SE- HPLC. The influence of detergents on association and dissociation of protein subunits and protein configuration is shown. These factors can affect the elution volume during chromatography. Furthermore it is shown that a direct comparison can be drawn between the protein separation by SE- HPLC on the one hand and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis on the other hand. As an example the separation of the δ- subunit of the acetylcholine receptor was shown under reducing and nonreducing conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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