Naslov
Membrane-active antimicrobial peptide identified in Rana arvalis by targeted DNA sequencing

Autori
Rončević, Tomislav ; Krce, Lucija ; Gerdol, Marco ; Pacor, Sabrina ; Benincasa, Monica ; Guida, Filomena ; Aviani, Ivica ; Čikeš-Čulić, Vedrana ; Pallavicini, Alberto ; Maravić, Ana ; Tossi, Alessandro

Izvornik
Biochimica et biophysica acta. Biomembranes (0005-2736) 1861 (2019), 3; 651-659

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antibacterial activity ; Aggregation ; Anuran antimicrobial peptides ; Membrane active peptides ; Targeted DNA sequencing

Sažetak
Antimicrobial peptides (AMPs) are naturally produced, gene encoded molecules with a direct antimicrobial activity against pathogens, often also showing other immune-related properties. Anuran skin secretions are rich in bioactive peptides, including AMPs, and we have reported a novel targeted sequencing approach to identify novel AMPs simultaneously in different frog species, from small quantities of skin tissue. Over a hundred full-length peptides were identified from specimens belonging to five different Ranidae frog species, out of which 29 were novel sequences. Six of these were selected for synthesis and testing against a panel of Gram-negative and Gram-positive bacteria. One peptide, identified in Rana arvalis, proved to be a potent and broad-spectrum antimicrobial, active against ATCC bacterial strains and a multi-drug resistant clinical isolate. CD spectroscopy suggests it has a helical conformation, while surface plasmon resonance (SPR) that it may self-aggregate/oligomerize at the membrane surface. It was found to disrupt the bacterial membrane at sub-MIC, MIC and above-MIC concentrations, as observed by flow cytometry and/or visualized by atomic force microscopy (AFM). Only a limited toxicity was observed towards peripheral blood mononuclear cells (PBMC) with a more pronounced effect observed against the MEC-1 cell line.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA