Pregled bibliografske jedinice broj: 988124
Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing // Bmc genomics, 19 (2018), 827, 10 doi:10.1186/s12864-018-5225-5 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 988124 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Autori
Rončević, Tomislav ; Gerdol, Marco ; Spazzali, Francesca ; Florian, Fiorella ; Mekinić, Stjepan ; Tossi, Alessandro ; Pallavicini, Alberto
Izvornik
Bmc genomics (1471-2164) 19
(2018);
827, 10
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Antimicrobial peptides ; Anura ; Innate immunity ; Parallel identification ; Signal peptide region
Sažetak
Background:Antimicrobial peptides (AMPs) are multifunctional effector molecules that often combine direct antimicrobial activities with signaling or immunomodulatory functions. The skin secretions of anurans contain a variety of such bioactive peptides. The identification of AMPs from frog species often requires sacrificing several specimens to obtain small quantities of crude peptides, followed by activity based fractionation to identify the active principles. Results:We report an efficient alternative approach to selectively amplify AMP-coding transcripts from very small amounts of tissue samples, based on RNA extraction and cDNA synthesis, followed by PCR amplification and high-throughput sequencing of size-selected amplicons. This protocol exploits the highly conserved signal peptide region of the AMP precursors from Ranidae, Hylidae and Bombinatoridae for the design of family- specific, forward degenerate primers, coupled with a reverse primer targeting the mRNA poly-A tail. Conclusions:Analysis of the assembled sequencing output allowed to identify more than a hundred full-length mature peptides, mostly from Ranidae species, including several novel potential AMPs for functional characterization.This (i) confirms the effectiveness of the experimental approach and indicates points for protocol optimization to account for particular cases, and (ii) encourages the application of the same methodology to other multigenic AMP families, also from other genera, sharing common features as in anuran AMPs.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija
POVEZANOST RADA
Projekti:
--IP-2013-11-8481 - Biofizikalni dizajn antimikrobnih peptida i inovativni molekularni deskriptori (BioAmpMode) (Vukičević, Damir) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Split
Profili:
Tomislav Rončević
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE