Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 988124

Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing


Rončević, Tomislav; Gerdol, Marco; Spazzali, Francesca; Florian, Fiorella; Mekinić, Stjepan; Tossi, Alessandro; Pallavicini, Alberto
Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing // Bmc genomics, 19 (2018), 827, 10 doi:10.1186/s12864-018-5225-5 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 988124 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing

Autori
Rončević, Tomislav ; Gerdol, Marco ; Spazzali, Francesca ; Florian, Fiorella ; Mekinić, Stjepan ; Tossi, Alessandro ; Pallavicini, Alberto

Izvornik
Bmc genomics (1471-2164) 19 (2018); 827, 10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antimicrobial peptides ; Anura ; Innate immunity ; Parallel identification ; Signal peptide region

Sažetak
Background:Antimicrobial peptides (AMPs) are multifunctional effector molecules that often combine direct antimicrobial activities with signaling or immunomodulatory functions. The skin secretions of anurans contain a variety of such bioactive peptides. The identification of AMPs from frog species often requires sacrificing several specimens to obtain small quantities of crude peptides, followed by activity based fractionation to identify the active principles. Results:We report an efficient alternative approach to selectively amplify AMP-coding transcripts from very small amounts of tissue samples, based on RNA extraction and cDNA synthesis, followed by PCR amplification and high-throughput sequencing of size-selected amplicons. This protocol exploits the highly conserved signal peptide region of the AMP precursors from Ranidae, Hylidae and Bombinatoridae for the design of family- specific, forward degenerate primers, coupled with a reverse primer targeting the mRNA poly-A tail. Conclusions:Analysis of the assembled sequencing output allowed to identify more than a hundred full-length mature peptides, mostly from Ranidae species, including several novel potential AMPs for functional characterization.This (i) confirms the effectiveness of the experimental approach and indicates points for protocol optimization to account for particular cases, and (ii) encourages the application of the same methodology to other multigenic AMP families, also from other genera, sharing common features as in anuran AMPs.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija



POVEZANOST RADA


Projekti:
--IP-2013-11-8481 - Biofizikalni dizajn antimikrobnih peptida i inovativni molekularni deskriptori (BioAmpMode) (Vukičević, Damir) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Split

Profili:

Avatar Url Tomislav Rončević (autor)

Poveznice na cjeloviti tekst rada:

doi bmcgenomics.biomedcentral.com

Citiraj ovu publikaciju:

Rončević, Tomislav; Gerdol, Marco; Spazzali, Francesca; Florian, Fiorella; Mekinić, Stjepan; Tossi, Alessandro; Pallavicini, Alberto
Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing // Bmc genomics, 19 (2018), 827, 10 doi:10.1186/s12864-018-5225-5 (međunarodna recenzija, članak, znanstveni)
Rončević, T., Gerdol, M., Spazzali, F., Florian, F., Mekinić, S., Tossi, A. & Pallavicini, A. (2018) Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing. Bmc genomics, 19, 827, 10 doi:10.1186/s12864-018-5225-5.
@article{article, author = {Ron\v{c}evi\'{c}, Tomislav and Gerdol, Marco and Spazzali, Francesca and Florian, Fiorella and Mekini\'{c}, Stjepan and Tossi, Alessandro and Pallavicini, Alberto}, year = {2018}, pages = {10}, DOI = {10.1186/s12864-018-5225-5}, chapter = {827}, keywords = {Antimicrobial peptides, Anura, Innate immunity, Parallel identification, Signal peptide region}, journal = {Bmc genomics}, doi = {10.1186/s12864-018-5225-5}, volume = {19}, issn = {1471-2164}, title = {Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing}, keyword = {Antimicrobial peptides, Anura, Innate immunity, Parallel identification, Signal peptide region}, chapternumber = {827} }
@article{article, author = {Ron\v{c}evi\'{c}, Tomislav and Gerdol, Marco and Spazzali, Francesca and Florian, Fiorella and Mekini\'{c}, Stjepan and Tossi, Alessandro and Pallavicini, Alberto}, year = {2018}, pages = {10}, DOI = {10.1186/s12864-018-5225-5}, chapter = {827}, keywords = {Antimicrobial peptides, Anura, Innate immunity, Parallel identification, Signal peptide region}, journal = {Bmc genomics}, doi = {10.1186/s12864-018-5225-5}, volume = {19}, issn = {1471-2164}, title = {Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing}, keyword = {Antimicrobial peptides, Anura, Innate immunity, Parallel identification, Signal peptide region}, chapternumber = {827} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font