Naslov
Changes in nanomechanical properties and adhesion dynamics of algal cells during their growth

Autori
Pillet, F ; Dague, E ; Pečar Ilić, Jadranka ; Ružić, Ivica ; Rols, M-P ; Ivošević DeNardis, Nadica

Izvornik
Bioelectrochemistry (Amsterdam) (1567-5394) 127 (2019); 154-162

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
AFM ; algal cell adhesion ; amperometry ; kinetics ; mathematical model ; nanomechanics

Sažetak
Nanomechanical and structural characterisations of algal cells are of key importance for understanding their adhesion behaviour at interfaces in the aquatic environment. We examine here the nanomechanical properties and adhesion dynamics of the algal cells during two phases of their growth using complementary surface methods and the mathematical modelling. Mechanical properties of motile cells are hard to assess while keeping cells viable, and studies to date have been limited. Immobilisation of negatively charged cells to a positively charged substrate enables high-resolution AFM imaging and nanomechanical measurements. Cells were stiffer and more hydrophobic in the exponential than in the stationary phase, suggesting molecular modification of the cell envelope during aging. The corresponding properties of algal cells were in agreement with the increase of critical interfacial tensions of adhesion, determined amperometrically. Cells in exponential phase possessed a larger cell volume, in agreement with the large amount of amperometrically measured displaced charge at the interface. Differences in the kinetics of adhesion and spreading of cells at the interface were attributed to their various volumes and nanomechanical properties that varied during cell aging. Our findings contribute to the present body of knowledge on the biophysics of algal cells on a fundamental level.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija, Interdisciplinarne prirodne znanosti, Elektrotehnika

Napomena
The Direction Générale de l’Armement (DGA) and the Midi-Pyrénées Région., Project PIERGEN ANR-12-ASTR-0039, the Agence Nationale de la Recherche (ANR), French Centre National de la Recherche Scientifique (CNRS)

POVEZANOST RADA