Naslov
Development of analytical method for LC-MS/MS determination of DON, 3-AcDON AND 15-AcDON in food

Autori
Ivešić, Martina ; Ladiš, Ana-Marija ; Krivohlavek, Adela ; Šikić, Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Archives of industrial hygiene and toxicology 69, Suppl. 1 / Šostar, Zvonimir ; Šikić, Sandra ; Krivohlavek, Adela - Zagreb : Institute For Medical Research and Occupational Health, 2018, 53-53

Skup
2. međunarodni kongres o sigurnosti i kvaliteti hrane = 2nd International Congress on Food Safety and Quality

Mjesto i datum
Opatija, Hrvatska, 13.11.2018. - 16.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cereals and cereal products ; contamination ; food safety ; masked forms ; mycotoxins

Sažetak
Mycotoxins are well known as metabolites, among others, of Fusarium fungi witch have a strong impact on human helath while consuming food. Deoxynivalenol (DON) as a mycotoxin can also be found in the acetylated forms (know as modified (masked) forms of DON: 3-Acetyldeoxynivalenol (3-AcDON) and 15-Acetyldeoxynivalenol (15- AcDON). DON acetylated forms may be found afer metabolization of some plants, animals, food production process and ect. Even though DON is constantly monitored in food, alertnes for 3- AcDON and 15-AcDON still isn't high enought. The reason for that may be interpreted in deficit of reliable analytical methods. The aim of this paper was development of analytical method for LC-MS/MS determination of DON, 3- AcDON and 15-AcDON. Samples of cereals and cereal products after homogenization were purified through immunoaffinity columns (IAC). Samples were contamination free from DON, 3- AcDON and 15-AcDON and spiked with different amounts of DON, 3-AcDON and 15-AcDON verifying suitability of developed method. The concentration amounts of DON, 3-AcDON and 15- AcDON in the samples were determined by high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Validation parameters of developed method (selectivity, recovery, precision, linearity, accuracy, limits of detection and quantification and robustness) were completely fulfilled. Sample recoveries were ranging from 62, 0 % up to 95, 5 % for concentrations 100 -1250 μg kg-1, limit of detection was 100 μg kg-1 while limit of quantification was 200 μg kg-1 for all analytes furthermore proving method is validated for determination of DON and it's acetylated forms in cereals and cereal products.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita

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