Naslov
Essential oil characterisation, antimicrobial and antioxidative activity of extracts of Erodium cicutarium (L.) L’Hér. ex Aiton (Geraniaceae) from Croatia

Autori
Ljoljić Bilić, Vanja ; Kosalec, Ivan ; Kremer, Dario ; Dunkić, Valerija ; Vuković Rodriguez, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
30th International Symposium on the Chemistry of Natural Products and the 10th International Congress on Biodiversity Abtract Book / - , 2018, 464-464

Skup
30th International Symposium on the Chemistry of Natural Products and the 10th International Congress on Biodiversity (ISCNP30 & ICOB10)

Mjesto i datum
Atena, Grčka, 25.11.2018. - 29.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Erodium cicutarium (L.) L’Hér. ex Aiton, essential oil, antioxidant capacity, antimicrobial activity.

Sažetak
The aim of this study was phytochemical characterisation of essential oil and screening of biological effects of plant extracts from aerial parts of Erodium cicutarium (L.) L’Hér. ex Aiton from four localities in Croatia (Buzin, Trešnjevka, Plitvice, Podvinje). This native traditional medicinal plant was used for essential oil preparation via hydrodistillation and GC-MS analysis was performed [1]. The screening of biological effects included in vitro antioxidant and antimicrobial activity evaluation of plant extracts (aqueous and methanolic). Cupric ion reducing antioxidant capacity assay (CUPRAC) was used to determine antioxidant potency [2]. Standard antimicrobial in vitro assays including agar well diffusion assay [3] and serial twofold microdilution assay [4], were used for an antimicrobial screening on Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536 and Candida albicans ATCC 90028. GC-MS analysis showed similarity in composition of the major compounds of all four essential oils and, in total, 50 compounds were identified with hydrocarbons as main class of constituents (62.5–65.7%). All analyzed extracts exhibited antioxidant activity in the CUPRAC assay, with higher values for methanol than water extracts. The most active extract was from locality Podvinje (74.6 mg TEs/g extract*). In the well diffusion assay, all investigated extracts show highest activity against S. aureus, with zones of bacterial growth inhibition (ZI) ranging from 12±2.5mm (Trešnjevka) to 21±1.41 mm (Podvinje). Activity against C. albicans was shown only for methanolic extracts, with highest value of ZI=15±2.55 mm (Podvinje). Within the microdilution assay, minimal inhibitory concentration (MIC) values were obtained only for S. aureus. MIC values are within the range from 3.75±1.77 mg/mL (Podvinje, Trešnjevka) to 20±0.00 mg/mL (Buzin). These results contribute to the scientific study of phytotherapeutic potential of the investigated plant species, especially in relations to essential oil composition, antioxidant and antimicrobial activity, and give an impetus for further biological studies. Note: *TEs/g extract, Trolox equivalents/g extract.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA