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Pregled bibliografske jedinice broj: 975329

Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys


Karaica, Dean; Breljak, Davorka; Lončar, Jovica; Lovrić, Mila; Micek, Vedran; Vrhovac Madunić, Ivana; Brzica, Hrvoje; Herak-Kramberger, Carol Mirna; Ivković Dupor, Jana; Ljubojević, Marija et al.
Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys // Arhiv za higijenu rada i toksikologiju, 69 (2018), 4; 286-303 doi:10.2478/aiht-2018-69-3157 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 975329 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys

Autori
Karaica, Dean ; Breljak, Davorka ; Lončar, Jovica ; Lovrić, Mila ; Micek, Vedran ; Vrhovac Madunić, Ivana ; Brzica, Hrvoje ; Herak-Kramberger, Carol Mirna ; Ivković Dupor, Jana ; Ljubojević, Marija ; Smital, Tvrtko ; Vogrinc, Željka ; Burckhardt, Gerhard ; Burckhardt, Birgitta Christina ; Sabolić, Ivan

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 69 (2018), 4; 286-303

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
anion exchanger ; immunolocalisation ; nephrolithiasis ; oxaluria ; RT-PCR ; sex differences ; urolithiasis

Sažetak
Chloride/formate exchanger (CFEX ; SLC26A6) mediates oxalate transport in various mammalian organs. Studies in Cfex knockout mice indicated its possible role in development of male-dominant hyperoxaluria and oxalate urolithiasis. Rats provide an important model for studying this pathophysiological condition, but data on Cfex (rCfex) localisation and regulation in their organs are limited. Here we applied the RT-PCR and immunochemical methods to investigate rCfex mRNA and protein expression and regulation by sex hormones in the pancreas, small intestine, liver, and kidneys from intact prepubertal and adult as well as gonadectomised adult rats treated with sex hormones. rCfex cDNA-transfected HEK293 cells were used to confirm the specificity of the commercial anti-CFEX antibody. Various biochemical parameters were measured in 24-h urine collected in metabolic cages. rCfex mRNA and related protein expression varied in all tested organs. Sex-independent expression of the rCfex protein was detected in pancreatic intercalated ducts (apical domain), small intestinal enterocytes (brush-border membrane ; duodenum > jejunum > ileum), and hepatocytes (canalicular membrane). In kidneys, the rCfex protein was immunolocalised to the proximal tubule brush-border with segment-specific pattern (S1=S2<S3), and both rCfex mRNA and protein expression exhibited male-dominant sex differences driven by stimulatory effects of androgens after puberty. However, urinary oxalate excretion was unrelated to renal rCfex protein expression. While the effect of male-dominant expression of rCfex in renal proximal tubules on urine oxalate excretion remains unknown, its expression in the hepatocyte canalicular membrane may be a pathway of oxalate elimination via bile.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-1481 - Starosno-ovisna ekspresija membranskih prijenosnika u štakora (AGEMETAR) (Sabolić, Ivan, HRZZ - 2013-11) ( CroRIS)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Klinički bolnički centar Zagreb

Poveznice na cjeloviti tekst rada:

doi hrcak.srce.hr

Citiraj ovu publikaciju:

Karaica, Dean; Breljak, Davorka; Lončar, Jovica; Lovrić, Mila; Micek, Vedran; Vrhovac Madunić, Ivana; Brzica, Hrvoje; Herak-Kramberger, Carol Mirna; Ivković Dupor, Jana; Ljubojević, Marija et al.
Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys // Arhiv za higijenu rada i toksikologiju, 69 (2018), 4; 286-303 doi:10.2478/aiht-2018-69-3157 (međunarodna recenzija, članak, znanstveni)
Karaica, D., Breljak, D., Lončar, J., Lovrić, M., Micek, V., Vrhovac Madunić, I., Brzica, H., Herak-Kramberger, C., Ivković Dupor, J. & Ljubojević, M. (2018) Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys. Arhiv za higijenu rada i toksikologiju, 69 (4), 286-303 doi:10.2478/aiht-2018-69-3157.
@article{article, author = {Karaica, Dean and Breljak, Davorka and Lon\v{c}ar, Jovica and Lovri\'{c}, Mila and Micek, Vedran and Vrhovac Maduni\'{c}, Ivana and Brzica, Hrvoje and Herak-Kramberger, Carol Mirna and Ivkovi\'{c} Dupor, Jana and Ljubojevi\'{c}, Marija and Smital, Tvrtko and Vogrinc, \v{Z}eljka and Burckhardt, Gerhard and Burckhardt, Birgitta Christina and Saboli\'{c}, Ivan}, year = {2018}, pages = {286-303}, DOI = {10.2478/aiht-2018-69-3157}, keywords = {anion exchanger, immunolocalisation, nephrolithiasis, oxaluria, RT-PCR, sex differences, urolithiasis}, journal = {Arhiv za higijenu rada i toksikologiju}, doi = {10.2478/aiht-2018-69-3157}, volume = {69}, number = {4}, issn = {0004-1254}, title = {Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys}, keyword = {anion exchanger, immunolocalisation, nephrolithiasis, oxaluria, RT-PCR, sex differences, urolithiasis} }
@article{article, author = {Karaica, Dean and Breljak, Davorka and Lon\v{c}ar, Jovica and Lovri\'{c}, Mila and Micek, Vedran and Vrhovac Maduni\'{c}, Ivana and Brzica, Hrvoje and Herak-Kramberger, Carol Mirna and Ivkovi\'{c} Dupor, Jana and Ljubojevi\'{c}, Marija and Smital, Tvrtko and Vogrinc, \v{Z}eljka and Burckhardt, Gerhard and Burckhardt, Birgitta Christina and Saboli\'{c}, Ivan}, year = {2018}, pages = {286-303}, DOI = {10.2478/aiht-2018-69-3157}, keywords = {anion exchanger, immunolocalisation, nephrolithiasis, oxaluria, RT-PCR, sex differences, urolithiasis}, journal = {Arhiv za higijenu rada i toksikologiju}, doi = {10.2478/aiht-2018-69-3157}, volume = {69}, number = {4}, issn = {0004-1254}, title = {Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys}, keyword = {anion exchanger, immunolocalisation, nephrolithiasis, oxaluria, RT-PCR, sex differences, urolithiasis} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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