Naslov
Cytogenetic Biomonitoring of Dental Technicians : A Cross-Sectional Study

Autori
Tadin, Antonija ; Gavić, Lidia ; Jurković, Ivana ; Vidović, Neven ; Jerković, Daniel ; Željezić, Davor

Izvornik
Journal of Prosthodontics : Implant Esthetic and Reconstructive Dentistry (1059-941X) 28 (2019), 2; 106-112

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Dental assistants ; Dentist ; DNA damage ; Micronucleus assay

Sažetak
This is a cross-sectional study to assess the incidence of micronuclei and other nuclear morphological changes in buccal epithelial cells of dental technicians. The study was conducted on 45 dental technicians vs. 2 control groups: 50 dentists and 50 dental assistants. DNA damage was analyzed in exfoliated buccal mucosa cells by micronucleus assay. The differences in the frequency of detected types of cytogenetic damage between experimental groups were analyzed by applying 2-way ANOVA with Tukey's HSD post-hoc test. Dentists and dental assistants have significantly lower incidence of micronucleated cells than technicians (mean ± SD: 0.68 ± 0.74, 0.58 ± 0.81, and 1.58 ± 2.07 ; p = 0.031 and p = 0.015, respectively), and this trend also holds for karyolitic cells (0.10 ± 0.30, 0.20 ± 0.49, and 1.42 ± 1.25 ; p < 0.001 and p < 0.001, respectively), condensed chromatin (0.16 ± 0.37, 0.14 ± 0.35, and 0.76 ± 0.98 ; p < 0.001 and p < 0.001, respectively), and pyknotic cells (0.04 ± 0.20, 0.08 ± 0.27, and 0.96 ± 1.24 ; p < 0.001 and p < 0.001, respectively). Cytogenetic biomarkers in dental technician buccal mucosa are increased compared with control groups. This increase may be associated with more extensive exposure to potentially harmful components of the materials used in everyday dental practice.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA