Naslov
Development and evaluation of an experimental protocol for 3-D visualization and characterization of the structure of bacterial biofilms in porous media using laboratory X-ray tomography

Autori
Ivanković, Tomislav ; Rolland du Roscoat, Sabine ; Geindreau, Christian ; Sechet, Philipe ; Huang, Zhuan ; Martins, Jean

Izvornik
Biofouling (0892-7014) 32 (2016), 10; 1235-1244

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biofilter ; biofilm ; X-ray tomography ; Pseudomonas putida

Sažetak
The development of a reliable model allowing accurate predictions of biofilm growth in porous media relies on a good knowledge of the temporal evolution of biofilm structure within the porous network. Since little is known about the real 3-D structure of biofilms in porous media, this work was aimed at developing a new experimental protocol to visualize the 3-D microstructure of the inside of a porous medium using laboratory X-ray microtomography. A reliable and reproducible methodology is proposed for (1) growing a biofilm inside a porous medium, and (2) X-ray tomography-based characterization of the temporal development of the biofilm at the inlet of the biofilter. The statistical analysis proposed here also validates the results presented in the literature based on a biofilm structure single measurement.

Izvorni jezik
Engleski

POVEZANOST RADA