Pregled bibliografske jedinice broj: 969246
A laboratory scale transglucosylation by using whole cells as biocatalysts
A laboratory scale transglucosylation by using whole cells as biocatalysts // European Summit of Industrial Biotechnology 2017 (esib 2017) / Berg, Gabriele et al. (ur.).
Graz: acib, 2017. str. 177-177 (poster, međunarodna recenzija, sažetak, ostalo)
CROSBI ID: 969246 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
A laboratory scale transglucosylation by using whole cells as biocatalysts
Autori
Vučenović, Ivan ; Slavica, Anita
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Izvornik
European Summit of Industrial Biotechnology 2017 (esib 2017)
/ Berg, Gabriele et al. - Graz : Acib, 2017, 177-177
Skup
European Summit of Industrial Biotechnology 2017 (esib 2017)
Mjesto i datum
Graz, Austrija, 14.11.2017. - 16.11.2017
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
whole-cells, transglucosylation, laboratory scale
Sažetak
Cultivation of L. mesenteroides, permeabilization of cultivated cells, and transglucosylation reaction catalyzed by the permeablized L. mesenteroides cells will be investigated. Cultivation parameters are to be optimized in order to achieve suitable growth of L. mesenteroides on sucrose without fructose conversion to mannitol and also production of other end-products of central metabolism (e.g. acetate and ethanol). Different physical and chemical methods will be screened in order to improve L. mesenteroides cell membrane permeability, like ultrasound treatment and addition of surfactants, and most efficient and sustainable method will be used for preparation of biocatalyst for the laboratory scale transglucosylation. In the whole-cells catalyzed reaction, sucrose and glycerol will be used as glucosyl donor and glucosyl acceptor, respectively, in order to synthesize 2-O-(α-D-glucopyranosyl)-sn-glycerol. Optimal ratio of the two reactants for an highly efficient transglucosylation reaction will be optimized. In a collaboration with a scientific partner we aim to optimize a detailed set of procedures for (i) the cultivation of L. mesenteroides, (ii) the permeabilization of cultivated cells, and (iii) the transglucosylation reaction catalyzed by the permeabilized L. mesenteroides cells. Different concentrations of the biocatalyst, donor and acceptor molecules, as well as a range of bioprocess parameters, primary pH and temperature, will be combined in order to increase transglucosylation efficiency at the laboratory scale.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
