Pregled bibliografske jedinice broj: 969237
Utilization of unusual substrates in lactic acid production by Lactobacillus amylovorus DSM 20531T
Utilization of unusual substrates in lactic acid production by Lactobacillus amylovorus DSM 20531T // European Summit of Industrial Biotechnology 2017 (esib 2017) / Berg, Gabriele et al. (ur.).
Graz: acib, 2017. str. 172-172 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 969237 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Utilization of unusual substrates in lactic acid production by Lactobacillus amylovorus DSM 20531T
Autori
Kolanović, Dajana ; Slavica, Anita
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
European Summit of Industrial Biotechnology 2017 (esib 2017)
/ Berg, Gabriele et al. - Graz : Acib, 2017, 172-172
Skup
European Summit of Insutrial BIotechnology 2017 (esib 2017)
Mjesto i datum
Graz, Austrija, 14.11.2017. - 16.11.2017
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
lactic acid, unusual substrates, Lactobacillus amylovorus
Sažetak
Disaccharide cellobiose [beta (1-4) glycosidic bond] can be natively transported into the cell by Lactobacillus amylovorus DSM 20531T and fermented with high percent theoretical yield to lactic acid being the main end-product (unpublished results). Besides, experimentally has been shown that the wild-type of this strain also uses maltose [alpha (1-4) glycosidic bond] and, after hydrolysis, higher maltooligosaccharides (unpublished data). Utilization of later substrates also resulted in a highly efficient production of different ratios of D- and L-lactic acid at mild temperature and maintained pH value in a laboratory-scale stirred tank reactor (up to 20 L). Glucose repression in all above cases was not observed. Collected data serves as a sound basis to build on in collaboration with a scientific and/or industrial partner in order to implement this versatile wild-type biocatalyst with GRAS status and adapt it for possible industrial applications. We offer a co-development of a bioprocess for D-/L-lactic acid production that will be catalized by the wild-type lactic acid bacterium strain L. amylovorus DSM 20531T capable of fermenting unusual substrates such as cellobiose. In addition, metabolic flexibility of the strain should be explored in terms of using other unusual substrates, e.g. xylose. Introduction of xylose specific transporter and xylose pathway in the wild-type strain may result in creation of an robust and functional biocatalyst to be selected for cascade processing of renewable feedstocks within a bioferinery.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
