Naslov
Remodeling of the intracellular compartments by cytomegalovirus

Autori
Blagojević Zagorac, Gordana ; Mahmutefendić Lučin, Hana ; Pavišić, Valentino ; Karleuša, Ljerka ; Marcelić, Marina ; Jug, Natalia ; Lukanovic Juric, Silvija ; Lučin, Pero

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS 2018 advanced course. The 2018 Golgi meeting: Membrane trafficking in cell organization and homeostasis. / - , 2018, 173-173

Skup
FEBS 2018 advanced course. The 2018 Golgi meeting: Membrane trafficking in cell organization and homeostasis.

Mjesto i datum
Sorrento, Italija, 15.10.2018. - 19.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MCMV, endocytosis, Arf proteins

Sažetak
Murine cytomegalovirus (MCMV) extensively reorganize membranous compartments of the host cell to form the virion assembly compartment (AC). The development of the AC starts very early in the infection with rearrangement of the Golgi network and endosomal recycling system. These rearrangements affect several intracellular processes required for viral pathogenesis. Inhibition of endosomal recycling is one of the earliest landmarks of MCMV infection. Reorganized endosomal recycling system intermix with fragmented Golgi elements, concentrate around the cell center and derives membranous structure that gather viral envelope and tegument proteins. These membranous organelle rearrangements were associated with altered expression and redistribution of small GTPases from Arf and Rab family that regulate early endosomal recycling circuit and endosome-to-Golgi trafficking, and altered recruitment of their effectors. MCMV infection increased expression of members of the Arf family (i.e., Arf1, Arf3, and Arf5), and increased membrane recruitment of their active forms (i.e., Arf1, Arf6) and their effectors (i.e., EPI64, Big2). In contrast reduced membrane recruitment of Rab35, its effectors ACAP2 (Arf6-GAPs) and MICAL-L1, and downstream Rabs (Rab8A, Rab15 and Rab13) indicate for overactivation of Arf6 and suggests for the alteration of the Arf6/Rab35 regulatory loop at the juxtanuclear endosomal aggregate. These results demonstrate that overactivation of Arf proteins, especially Arf6, may represent the earliest alteration which leads to the reorganization of the endosomal recycling system and the Golgi in MCMV infected cells. Understanding these processes during the MCMV infection may enable understanding the virion assembly-associated processes during herpesvirus infection and the development of therapeutic strategies.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA