Naslov
Antiproliferative effect of novel coumarin-1, 2, 3-triazole hybrids against tumor cells in vitro

Autori
Jukić, Marijana ; Opačak-Bernardi, Teuta ; Živković, Zorislava ; Matić, Matea ; Bistrović, Andrea ; Stipaničev, Nikolina ; Raić-Malić, Silvana ; Glavaš-Obrovac, Ljubica.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDIR-5:˝Translating Science to Medicine - Targets and Therapeutics˝ / - , 2018, 56-56

Skup
Fifth Meeting of the Croatian Association for Cancer Research with International Participation

Mjesto i datum
Zagreb, Hrvatska, 08.11.2018. - 10.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
Coumarins, antiproliferative, tumor cells

Sažetak
Coumarins constitute a large family of oxygenated heterocyclic secondary metabolites widely distributed in plants. Natural and synthetic coumarins have attracted interest of scientist because of their broad spectrum of pharmacological activities among which are acting as an antioxidants and antitumor agents. Novel of coumarin-1, 2, 3-triazole hybrids were designed to evaluate their antiproliferative effect against carcinoma cells (HeLa, CaCo-2, SW620 and HT29), leukemia cells (K562 and CCRF-CEM), lymphoma cells (Raji and HuT-78), and MDCK as a normal cell line. Antiproliferative effect on coumarins hybrids was estimated after 72h by MTT test. Membrane integrity, as an indicator of cytotoxicity of selected hybrids was determined after 3, 6, 12 and 24 h by LDH release assay. The antioxidant capacity of new coumarine hybrids was determined by DPPH scavenging during 1h. Flow cytometry was used to determine cell cycle, as well as detection of ROS and apoptosis in K562 cells. Among tested coumarin hybrids, 6, 7 – dihydroxycoumarins showed the highest antioxidative activity with meaningful cell growth inhibition of tumor cells. Disrupted cell membrane integrity is apparent after 3h of incubation with >25% of LDH released. Tested hybrids caused accumulation of cells mostly in G1 and G2/M phases in cell cycle. One of tested dihydroxycoumarin showed reduction of ROS accumulation in K562 cells. Induction of apoptosis in K562 cells was not detected. Obtained results suggest that further biological evaluation of new synthesized coumarines hybrids is required.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

POVEZANOST RADA