Naslov
UPLC‐MS2 Profiling of Blackthorn Flower Polyphenols Isolated by Ultrasound‐Assisted Extraction

Autori
Elez Garofulić, Ivona ; Zorić, Zoran ; Pedisić, Sandra ; Brnčić, Mladen ; Dragović-Uzelac, Verica

Izvornik
Journal of food science (0022-1147) 83 (2018), 11; 2782-2789

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
blackthorn flowers, polyphenols, RSM, ultrasound-assisted extraction, UPLC-MS2

Sažetak
Ultrasound-assisted extraction (UAE) was optimized for fast and effective isolation of blackthorn flower polyphenols. The effects of ethanol concentration (50 and 70 %) in extraction solvent, ultrasound amplitude (50, 75 and 100 %) and extraction time (3, 6 and 9 min) were evaluated. UPLC-MS2 was used to determine phenolic profile of blackthorn flowers extracts. A total of 28 different phenolic compounds were identified, belonging to the classes of hydroxycinnamic acids, flavonol glycosides and flavanols. Kaempferol and quercetin derivatives were the most abundant compounds represented by kaempferol- pentoside and rhamnoside in concentrations up to 494.94 and 436.62 mg/100 g and quercetin- pentoside in concentration up to 226.75 mg/100 g. Observed parameters of UAE did not affect the qualitative phenolic composition of blackthorn flower extracts. On the contrary, the concentration of polyphenols increased with higher ethanol concentration in solvent, higher ultrasound amplitude and prolongation of extraction time. The established conditions for optimal extraction of blackthorn flower polyphenols using ultrasound were: 70 % ethanol as extraction solvent, 75 % of ultrasound amplitude and 9 min extraction time, resulting in extract rich in polyphenols with a great potential for use in pharmaceutical and food industry.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA