Naslov
An insight into the toxic effects of irinotecan and delta 9-tetrahydrocannabinol through measurements of cholinesterase activities and markers of oxidative stress in rat plasma

Autori
Mikolić, Anja ; Žunec, Suzana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Pokusne životinje u znanstvenim istraživanjima - knjiga sažetaka / - Zagreb, 2018, 43-43

Skup
3. znanstveno-stručni simpozij Hrvatskog društva za znanost o laboratorijskim životinjama ; 2. zajednički skup CroLASA-e i SLAS-a s međunarodnim sudjelovanjem = 3rd Congress of Croatian Laboratory Animal Science Association ; 2nd joint Congress of CroLASA and SLAS with international participation

Mjesto i datum
Zagreb, Hrvatska, 25.10.2018. - 26.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
irinotecan, delta 9-tetrahydrocannabinol, rats, cholinesterases, oxidative stress

Sažetak
Irinotecan (IRI) is one of the most important antineoplastic drugs primarily intended for use in chemotherapy of metastatic colorectal cancer. Patients treated with IRI often manifest acute cholinergic syndrome, which is the reason behind the growing use of legally prescribed preparations that contain cannabinoids as well as illicit ones that sometimes contain very high delta 9-tetrahydrocannabinol (THC) levels. The objective of this pilot study was to evaluate the toxic effects of a concomitant intake of IRI and THC in male Wistar rats. IRI was administered once intraperitoneally (at 100 mg/kg b.w.) and THC per os repeatedly for 1, 3, and 7 days (at 7 mg/kg b.w.). The rats were sacrificed 24 h after the last treatment. The plasma samples were collected and stored for biochemical analyses. IRI either alone or in combination with THC inhibited acetylcholinesterase activity by 30 and 20 % according to control after 1 and 3 days. Repeated application of THC for 3 and 7 days resulted with inhibition of butyrylcholinesterase activity. Although IRI and THC treatments generally did not affect biomarkers of oxidative stress (lipid peroxidation, superoxide dismutase and catalase), an increase of total antioxidant capacity in the plasma of rats treated with THC was noticed after 1 and 7 days. The biomarkers used in this study provide a static assessment of the adverse effects of IRI and THC in rats so future research should focus on evaluating their interactions by molecular methods.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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