Naslov
Development of new functional supramolecular systemsbased on β-cyclodextrin and liposomes for gene delivery
(Development of new functional supramolecular systemsbased onβ-cyclodextrin and liposomes for gene delivery)

Autori
Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Frkanec, Leo ; Šekutor, Marina ; Frkanec, Ruža

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14th Greta Pifat Mrzljak International School of Biophysics. ABC of Physics of Life: Book of abstracts / Delač Marion, Ida ; Vuletić, Tomislav - Zagreb : Institut Ruđer Bošković ; Hrvatsko biofizičko društvo, 2018, 119-119

ISBN
978-953-7941-24-6

Skup
14th Greta Pifat Mrzljak International School of Biophysics: ABC of physics of life

Mjesto i datum
Split, Hrvatska, 23.08.2018. - 01.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Adamantyl guanidine ; cyclodextrine ; supramolecular complexes, liposomes, host-guest interaction, fluorescence correlation spectroscopy ; gene delivery

Sažetak
The aim of the present study was preparation and characterization of supramolecularsystems with adamantyl guanidine (AG) and probing of their interaction with DNA usinguorescence correlation spectroscopy (FCS). In the continuation of our study dealing withadamantyl guanidines and investigations of lipid-based nanovesicles as drug delivery systems[1, 2] we prepared and characterized functionalized supramolecular systems of AG 1-5 withphosphatidylcholine liposomes, liposomes incorporating amphiphilicβ- cyclodextrin (β-CD)derivative and cyclodextrin vesicles composed only from the amphiphilicβ-CD derivative.Eciency of incorporation of AG 1-5 into prepared supramolecular systems was measuredspectrophotometrically, and surface charge were determined by dynamic light scatteringmethod. FCS was applied to examine the interactions between functionalized supramolecularsystems and the Cy5 uorescently labelled, double-stranded, 120 bp DNA (DNA120*). Theobtained results have clearly demonstrated that the supramolecular systems with entrappedAG 1-4 present guanidinium groups on the surface of vesicles, which leads to interactionwith DNA120* via guanidine- phosphate interaction. Preliminary results have shown thatprepared functional supramolecular systems strongly bind DNA and as such could be usedin gene delivery.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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