Naslov
Cyclodextrin based supramolecular systems functionalized with adamantyl guanidines for gene delivery: preparation, characterization and testing the interaction with DNA

Autori
Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Frkanec, Leo ; Šekutor, Marina ; Frkanec, Ruža

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th Regional Biophysics Conference : Book of Abstracts / Majaron, Hana ; Čotar, Petra ; Koren, Monika ; Golmajer Zima, Neža - Ljubljana : Slovenian Biophysical Society, 2018, 141-141

ISBN
978-961-90942-4-2

Skup
8th Regional Biophysics Conference (RBC2018)

Mjesto i datum
Zreče, Slovenija, 16.05.2018. - 20.05.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
supramolecular systems ; liposomes ; vesicles ; DNA ; fluorescence correlation spectroscopy ; FCS

Sažetak
The aim of the present study was preparation and characterization of supramolecular systems with adamantyl guanidine (AG) and probing of their interaction with DNA using fluorescence correlation spectroscopy (FCS). In the continuation of our study dealing with adamantyl guanidines and investigations of lipid-based nanovesicles as drug delivery systems (1, 2) we prepared and characterized functionalized supramolecular systems of adamantyl guanidines AG 1-5 with phosphatidylcholine liposomes, liposomes incorporating amphiphilic -cyclodextrin (-CD) derivative and cyclodextrin vesicles composed only from the amphiphilic -CD derivative. Efficiency of incorporation of AG 1-5 into prepared supramolecular systems was measured spectrophotometrically, and the size and surface charge were determined by dynamic light scattering method. The incorporation of AG 1-5 did not affect the size of prepared supramolecular systems but surface charge was significantly changed. FCS was applied to examine the interactions between functionalized supramolecular systems and the Cy5- fluorescently labelled, double-stranded, 120 bp DNA (DNA120*). The obtained results have clearly demonstrated that the supramolecular systems with entrapped AG 1-4 present guanidinium groups on the surface of vesicles, which leads to interaction with DNA120* via guanidine-phosphate interaction. Preliminary results have shown that prepared functional supramolecular systems strongly bind DNA and as such could be used in gene delivery.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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