Naslov
Intensive exercise induces an increase in anti- inflammatory IgG N-glycans

Autori
Tijardović, Marko ; Keser, Toma ; Kifer, Domagoj ; Marijančević, Domagoj ; Lauc, Gordan ; Gornik, Olga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd GlycoCom 2018 and 1st Human Glycome Project Meeting book of abstracts / - , 2018, 109-110

Skup
2nd GlycoCom 2018 and 1st Human Glycome Project Meeting

Mjesto i datum
Dubrovnik, Hrvatska, 03.10.2018. - 06.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
IgG ; inflammation ; exercise

Sažetak
As one of the most important posttranslational protein modifications, glycosylation promises to be a useful tool in prediction and prognosis of different diseases. Because glycans are affected by the change in physiological parameters, they could be used to assess the general health status of an individual. It is known that physical exercise initiates a cascade of inflammatory events, which over time lead to long term beneficial anti-inflammatory effects on human body. Since there is a connection between glycans and inflammation we wanted to explore if strenuous physical activity will manifest itself as visible changes in glycan profiles. Two male groups comprised of first-year kinesiology students were involved in the study. The experimental group (N = 15) underwent an intense repeated sprint training for two months, while the control group (N = 14) was refrained from doing organised physical exercise. Blood plasma samples from both groups were taken in three timepoints: before training began, after the most intense part of the program and one month after the program ended. We analysed total plasma N-glycome and IgG N-glycome from each sample. Glycans were released from glycoproteins using PNGase F, labeled with 2-AB fluorescent dye and analysed by a HILIC-UPLC method. Using mixed model regression analysis, plasma N-glycans did not show any significant changes between groups and timepoints, but by analysing IgG N-glycans we detected a change in the experimental group's last timepoint. One month after the end of the exercise program, di-galactosylated glycans (p = 0.012) and mono- sialylated glycans (p = 0.037) levels were higher than at the beginning of the training. Rise in these two glycan groups is known to be linked with anti-inflammatory effects of IgG, which leads us to the conclusion that intense training could have resulted in compensatory anti-inflammatory mechanisms as a response to exercise-induced stress.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA