Naslov
Estimation of the withdrawal time of levamisole in eggs after oral administration to laying hens

Autori
Solomun Kolanović, Božica ; Bilandžić, Nina ; Varenina, Ivana ; Božić Luburić1, Đurđica ; Varga, Ines ; Cvetnić, Luka ; Benić, Miroslav ; Cvetnić, Željko ; Denžić Lugomer, Marija ; Pavliček, Damir ; Šušković, Jagoda ; Kos, Blaženka

Izvornik
Journal of food protection (0362-028X) 81 (2018), 10; 1627-1634

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
levamisole ; residues ; eggs ; depletion ; withdrawal time ; mass spectrometry

Sažetak
This study was conducted to evaluate withdrawal time of levamisole in eggs following oral administration in laying hens at different doses. Sampling of eggs was conducted for 37 days following the end of treatment and levamisole concentrations were measured by liquid chromatography mass spectrometry method (LC-MS/MS) validated according to the Commission Decision 2002/657/EC. Estimated validation parameters were: decision limit (CCα) 0.54 μg/kg, detection capability (CCβ) 0.56 μg/kg, limit of detection (LOD) 0.04 μg/kg, limit of quantification (LOQ) 0.15 μg/kg, accuracy (recovery) between 92.9 and 102.3%, precision (relative standard deviation, RSD) ≤ 4.62 % and within-laboratory precision (RSD) ≤ 5.19%. Levamisole residue levels were three times higher in egg yolks than in egg whites. The highest levels of levamisole were detected on day 2 post-treatment in group receiving 50 mg/kg body weight (556.2 μg/kg in egg yolk and 166.5 μg/kg in egg white). Significant elimination occurred within 5 days after the cessation of treatment in all groups, with elimination half-life of 1.3 days. Levamisole was still detectable on day 30 after the end of treatment in egg white (0.06 μg/kg) and on day 37 in egg yolks (0.06 μg/kg). The longest withdrawal time for levamisole in eggs (14.9 days) was determined in a group treated with the recommended dose for two consecutive days. According to the results, oral treatment of laying hens with levamisole may result in non-compliant egg samples even 14 days after treatment.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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