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Pregled bibliografske jedinice broj: 958835

Mass spectrometry-based investigation of measles and mumps virus proteome


Sviben, Dora; Forčić, Dubravko; Halassy, Beata; Allmaier, Guenter; Marchetti- Deschmann, Martina; Brgles, Marija
Mass spectrometry-based investigation of measles and mumps virus proteome // Virology journal, 15 (2018), 160, 14 doi:10.1186/s12985-018-1073-9 (međunarodna recenzija, članak, znanstveni)


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Naslov
Mass spectrometry-based investigation of measles and mumps virus proteome

Autori
Sviben, Dora ; Forčić, Dubravko ; Halassy, Beata ; Allmaier, Guenter ; Marchetti- Deschmann, Martina ; Brgles, Marija

Izvornik
Virology journal (1743-422X) 15 (2018); 160, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
measles virus, mumps virus, extracellular vesicles, host cell proteins, mass spectrometry

Sažetak
Background: Measles (MEV) and mumps virus (MUV) are enveloped, non-segmented, negative single stranded RNA viruses of the family Paramyxoviridae, and are the cause of measles and mumps, respectively, both preventable by vaccination. Aside from proteins coded by the viral genome, viruses are considered to contain host cell proteins (HCPs). The presence of extracellular vesicles (ECVs), which are often co-purified with viruses due to their similarity in size, density and composition, also contributes to HCPs detected in virus preparations, and this has often been neglected. The aim was to identify which virus- coded proteins are present in MEV and MUV virions, and to try to detect which HCPs, if any, are incorporated inside the virions or adsorbed on their outer surface, and which are more likely to be a contamination from co- purified ECVs. Methods: MUV, MEV and ECVs were purified by ultracentrifugation, hydrophobic interaction chromatography and immunoaffinity chromatography, proteins in the samples were resolved by SDS-PAGE and subjected to identification by MALDI-TOF/TOF-MS. A comparative analysis of HCPs present in all samples was carried out. Results: By proteomics approach, it was verified that almost all virus-coded proteins are present in MEV and MUV particles. Proteins C in MEV which was until now considered to be non-structural viral protein, was found to be present inside the MeV virions. Results on the presence of HCPs in differently purified virus preparations imply that actin, annexins, cyclophilin A, moesin and integrin β1 are part of the virions. Conclusions: All HCPs detected in the viruses are present in ECVs as well, indicating their possible function in vesicle formation, or that most of them are only present in ECVs. Only five HCPs were constantly present in purified virus preparations, regardless of the purification method used, implying they are likely the integral part of the virions. The approach described here is helpful for further investigation of HCPs in other virus preparations.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
HRZZ-UIP-2013-11-8193 - Kromatografsko pročišćavanje biomolekula i njihova karakterizacija (CHROBIO) (Brgles, Marija, HRZZ - 2013-11) ( CroRIS)

Ustanove:
Sveučilište u Zagrebu

Profili:

Avatar Url Beata Halassy (autor)

Avatar Url Marija Brgles (autor)

Avatar Url Dubravko Forčić (autor)

Poveznice na cjeloviti tekst rada:

doi virologyj.biomedcentral.com

Citiraj ovu publikaciju:

Sviben, Dora; Forčić, Dubravko; Halassy, Beata; Allmaier, Guenter; Marchetti- Deschmann, Martina; Brgles, Marija
Mass spectrometry-based investigation of measles and mumps virus proteome // Virology journal, 15 (2018), 160, 14 doi:10.1186/s12985-018-1073-9 (međunarodna recenzija, članak, znanstveni)
Sviben, D., Forčić, D., Halassy, B., Allmaier, G., Marchetti- Deschmann, M. & Brgles, M. (2018) Mass spectrometry-based investigation of measles and mumps virus proteome. Virology journal, 15, 160, 14 doi:10.1186/s12985-018-1073-9.
@article{article, author = {Sviben, Dora and For\v{c}i\'{c}, Dubravko and Halassy, Beata and Allmaier, Guenter and Marchetti- Deschmann, Martina and Brgles, Marija}, year = {2018}, pages = {14}, DOI = {10.1186/s12985-018-1073-9}, chapter = {160}, keywords = {measles virus, mumps virus, extracellular vesicles, host cell proteins, mass spectrometry}, journal = {Virology journal}, doi = {10.1186/s12985-018-1073-9}, volume = {15}, issn = {1743-422X}, title = {Mass spectrometry-based investigation of measles and mumps virus proteome}, keyword = {measles virus, mumps virus, extracellular vesicles, host cell proteins, mass spectrometry}, chapternumber = {160} }
@article{article, author = {Sviben, Dora and For\v{c}i\'{c}, Dubravko and Halassy, Beata and Allmaier, Guenter and Marchetti- Deschmann, Martina and Brgles, Marija}, year = {2018}, pages = {14}, DOI = {10.1186/s12985-018-1073-9}, chapter = {160}, keywords = {measles virus, mumps virus, extracellular vesicles, host cell proteins, mass spectrometry}, journal = {Virology journal}, doi = {10.1186/s12985-018-1073-9}, volume = {15}, issn = {1743-422X}, title = {Mass spectrometry-based investigation of measles and mumps virus proteome}, keyword = {measles virus, mumps virus, extracellular vesicles, host cell proteins, mass spectrometry}, chapternumber = {160} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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