Naslov
Anti-tumor Effects of Newcastle Disease Virus in Mouse Mammary Carcinoma and Ehrlich Ascites Tumor

Autori
Gaćina, Lydia ; Odeh, Dyana ; Oršolić, Nada ; Kukolj, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Immuno-Oncology 2018 World Congress / - , 2018, 57-57

Skup
Immuno-Oncology 2018 World Congress

Mjesto i datum
Beč, Austrija, 24.06.2018. - 25.06.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Newcastle disease, mouse mammary carcinoma, Ehrlich ascites tumor

Sažetak
Oncolytic viruses (OVs) selectively replicate in and kill cancer cells, spread within the tumor, while not harming normal tissue, and are also very effective at inducing immune responses to themselves and to the infected tumor cells. This study investigated the anti- tumor effect of the lentogenic LaSota and B1 strains of Newcastle disease virus (NDV) against mouse mammary carcinoma (MCa) and Ehrlich ascites tumor (EAT). The aim was to determine the anti-tumor efficacy of these viruses themselves using in vitro cell culture models, or in combined effect with cytostatic cisplatin and hyperthermia using in vivo experimental model of lung metastases of MCa and EAT model. We injected Swiss albino mice with 2 x 106 EAT cells intraperitoneally (i.p.). The experimental groups were treated 48 hours after the introduction of EAT cells and after the spontaneous development of MCa i.p. and i.v. (intravenous) with 19 x 105 EID50 virus strains LaSota or B1 alone or in combination with cytostatic cisplatin (10 mg/kg body weight) in physiological conditions (37 °C) or the hyperthermal conditions (43 °C). The administered viruses showed a significant anti- tumor effect against MCa and the treated groups had significantly fewer lung metastases than the controls. The i.p. and i.v. administered viruses entered the bloodstream and produced viraemia, thus infecting the circulating tumor cells and tissue cells to achieve an oncolytic effect. At physiological temperature, both viruses achieved a strong anti-tumor effect, while in intraperitoneal hyperthermia they produced no effect. Cisplatin significantly increased the effect of the viruses both at the physiological temperature and in hyperthermia. The results of this study show that the NDV LaSota and B1 strains have a significant anti- tumor effect against MCa and EAT in vitro and in vivo. Cisplatin shows it can be an effective cytotoxic agent in combination with viruses against these tumors.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA