Naslov
Optimization of ultrasound-assisted extraction of biomass from olive trees using response surface methodology

Autori
Martínez-Patiño, José Carlos ; Gullón, Beatriz ; Romero ; Ruiz, Encarnación ; Brnčić⁠, Mladen ; Šic Žlabur, Jana ; Castro, Eulogio

Izvornik
Ultrasonics sonochemistry (1350-4177) 51 (2019); 487-495

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Extraction from biomass ; Olive tree pruning biomass ; Olive mill leaves ; Antioxidant activity ; Biorefinery

Sažetak
Olive tree pruning biomass (OTP) and olive mill leaves (OML) are the main residual lignocellulosic biomasses that are generated from olive trees. They have been proposed as a source of value-added compounds and biofuels within the biorefinery concept. In this work, the optimization of an ultrasound-assisted extraction (UAE) process was performed to extract antioxidant compounds present in OTP and OML. The effect of the three parameters, ethanol/water ratio (20, 50, 80% of ethanol concentration), amplitude percentage (30, 50, 70%) and ultrasonication time (5, 10, 15min), on the responses of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities (DPPH, ABTS and FRAP) were evaluated following a Box–Behnken experimental design. The optimal conditions obtained from the model, taking into account simultaneously the five responses, were quite similar for OTP and OML, with 70% amplitude and 15min for both biomasses and a slight difference in the optimum concentration of ethanol. (54.5% versus 51.3% for OTP and OML, respectively). When comparing the antioxidant activities obtained with OTP and OML, higher values were obtained for OML (around 40% more than for OTP). The antioxidant activities reached experimentally under the optimized conditions were 31.6mg of TE/g of OTP and 42.5mg of TE/g of OML with the DPPH method, 66.5mg of TE/g of OTP and 95.9mg of TE/g of OML with the ABTS method, and 36.4mg of TE/g of OTP and 49.7mg of TE/g of OML with the FRAP method. Both OTP and OML could be a potential source of natural antioxidants.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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