Naslov
Critical evaluation of the use of TXRF for the analysis of whole blood samples: Application to patients with thyroid gland diseases

Autori
Margui, Eva ; Jablan, Jasna ; Gerić, Marko ; Inić, Suzana ; Domijan, Ana-Marija ; Janušić, Renato ; Šarčević, Božena ; Queralt, Ignasi ; Garaj-Vrhovac, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts European Conference on X-Ray Spectrometry / Kavčić, Matjaž ; Žitnik, Matjaž ; Hrast, Mateja ; Bučar, Klemen - Ljubljana, 2018, 246-246

ISBN
978-961-264-126-9

Skup
European Conference on X-Ray Spectrometry (EXRS2018)

Mjesto i datum
Ljubljana, Slovenija, 24.06.2018. - 29.06.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
TXRF, multielement analysis, blood, thyroid gland diseases

Sažetak
Multielement analysis of whole blood can provide significant information for the evaluation of nutritional status, diagnosis of certain diseases as well as for the assessment of exposure to potentially toxic metals. However, the quantification of multiple elements in whole blood is not easy partly because of the wide variation in element concentrations (from ng/L to g/L) and the complex matrix. Since the publication by Prange et al. [1], total reflection X-ray fluorescence (TXRF) has been established as an effective tool for the analysis of trace elements in blood. However, most of the contributions dealing with this topic apply a previous digestion of the blood sample before the TXRF analysis. The aim of this work was to develop a fast, sustainable and reliable analytical method, in combination with low power TXRF, for multielement analysis of whole blood samples. For that, dilution is proposed as a sample treatment alternative. Firstly, a set of experiments were carried out to select the best diluent type and dilution factor using the control material SeronormTM Trace Elements Whole Blood L-1. Results obtained showed that the best strategy to prepare the sample was a dilution 1:5 with Triton 1% (in water). A critical evaluation of the parameters affecting the sample deposition on the reflector (i.e. sample deposition volume and drying mode) was also carried out including a study of the shape and element distribution of the deposited residue on the reflector by micro X-ray fluorescence spectrometry. It was demonstrated that using a sample deposition volume of 10 μL and drying the drop under IR radiation a centred thin film is obtained which is the basis to perform the analysis under total reflection conditions. Using the best analytical conditions and a measuring time of 2000s, limits of detection estimated were in the low mg/kg range (Ca, K: 14-25 mg/kg, Fe, Cu, Zn, Br, Rb, Cr, Ni, As, Se, Ag, Cd, Sn, Sb, Pb: 0.2-1.3 mg/kg) and similar to those obtained using more complex sample treatments such as digestion. Accuracy and precision of the results, evaluated by the analysis of the aforementioned blood control material, were in most cases acceptable (Recoveries: 89-102 %, RSD: 6-8%, n=5). To prove the applicability of the method, several blood samples from control and thyroid diseases patients were analysed. Despite the fact that more samples need to be analysed, it seems that Zn and Br contents in some of the patients with thyroid diseases are significantly higher compared to control samples.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA