Naslov
Assessing toxicity potential of cerium oxide nanoparticles towards developing embryos ; impact on three Mediterranean sea urchin species Arbacia lixula, Paracentrotus lividus and Sphaerechinus granularis

Autori
Burić, Petra ; Alač, Iva ; Lyons, Daniel Mark

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / - , 2018, 335-336

Skup
13. Hrvatski biološki kongres

Mjesto i datum
Poreč, Hrvatska, 19.09.2018. - 23.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
nanočestice cerijeva dioksida ; embrionalni razvoj ježinaca ; Arbacia lixula ; Paracentrotus lividus ; Sphaerechinus granularis
(cerium oxide nanoparticles ; sea urchin embryonal development ; Arbacia lixula ; Paracentrotus lividus ; Sphaerechinus granularis)

Sažetak
Cerium dioxide nanoparticles (CeO2NP) have recently found use in a wide range of applications including functional coatings, electronics and as fuel additives. It is the latter which may be of particular concern as the environmental load deriving from automobile exhaust is expected to be significant. Herein, the potential toxicity of CeO2NP was investigated using the sea urchin embryonal development test (SUEDT) with three sea urchin species Arbacia lixula, Paracentrotus lividus and Sphaerechinus granularis. Sea urchin sperm were treated with CeO2NP at concentrations ranging between 0.01-10 mg/L. Results indicated that CeO2NP did not affect the fertilization ability of sea urchin sperm with, more than 90% of eggs for each species successfully fertilized. These eggs developed into morphologically normal larvae at all NP concentrations except for the 10 mg/L CeO2NP treatment where larvae of A. lixula and P. lividus showed only 66.8 ± 8.4 and 82.3 ± 3.4 % normally developed larvae after 48h, respectively. Embryonal development following exposure of zygotes to CeO2NP at 30 min post fertilisation proceeded for all three species without significant differences to the control. Thus, SUEDT, as a standardized test, did not indicate that the investigated CeO2NP is a material of concern, even at extremely high concentrations. However, more research is required based on other models, particularly lung models, before concerns about nanoparticulate ceria may be completely alleviated.

Izvorni jezik
Engleski

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