Naslov
Mesenchymal stem cells and chondrocyte co - culture promote chondrocyte matrix production in normoxic conditions
(Mesenchymal stem cells and chondrocyte co - culture promote chondrocyte matrix production in normoxic conditions)

Autori
Antunovic, Maja ; Jagric, Mihaela ; Pribolsan, Lidija ; Panek, Marina ; Verbanac, Donatella ; Matic Igor ; Caput Mihalic, Katarina ; Marijanovic, Inga.

Izvornik
Molecular and Experimental Biology in Medicine (MEBM) (2584-671X) 1 (2018), 2; 12-17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chondrocytes ; mesenchymal stem cells ; tissue engineering ; peptide hydrogel ; cartilage

Sažetak
Articular cartilage is a tissue that shows no capacity for effective spontaneous regeneration. Tissue engineering, as a new important field of regenerative medicine, emerges as potential effective solution for treatment of articular cartilage injuries and defects. The aim of this study was to evaluate the chondrocyte preference of oxygen level for three-dimensional (3D) in vitrochondrogenesis and to test whether the presence of mesenchymal stem cells (MSCs) would influence the preference of oxygen level. In the experiment, we set up two different cell cultures: only chondrocytes and combination of chondrocytes and MSCs in 2:1 ratio. Cells were grown in a chondrogenic medium, incorporated in a peptide hydrogel RADA, in either normoxic (20% O2) or hypoxic (5% O2) conditions. After 19 days, expression levels of two cartilage marker genes, SOX9and ACAN, were evaluated by real-time quantitative PCR. In conclusion, hypoxia enhanced chondrogenesis significantly only in the case of chondrocytes, while co-culturing with MSCs slightly improved differentiation in normoxic conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Temeljne medicinske znanosti

POVEZANOST RADA