Naslov
Plant aminoacyl-tRNA synthetases in abiotic stress

Autori
Baranasic, Jurica ; Kekez, Mario ; Mihalak, Anita ; Jasmina, Rokov Plavec

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS3+ From molecules to living systems / Dávid Szüts, Laszlo Buday - Veszprém, 2018, 114-114

ISBN
978-615-5270-47-5

Skup
FEBS3+ conference "From molecules to living systems"

Mjesto i datum
Siófok, Mađarska, 02.09.2018. - 05.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
plant aminoacyl-tRNA synthetases ; abiotic stress ; RT-qPCR

Sažetak
Aminoacyl-tRNA synthetases (AARS) are essential enzymes that covalently link amino acid and tRNA. Aminoacylated tRNAs are substrates for protein translation on ribosomes. Besides their central role in translation, AARSs from various organisms participate in other essential cellular processes. However, there are only two reports on noncanonical functions of plant AARSs. Arabidopsis aspartyl-tRNA synthetase and rice methionyl-tRNA synthetase are involved in plant defense mechanisms against pathogen attack. In order to reveal novel noncanonical functions of plant AARSs, we determined the expression levels of selected AARS genes in Arabidopsis thaliana seedlings exposed to various abiotic stress conditions (high heavy metal concentration (cadmium), salinity and osmotic stress) using RT- qPCR. In another set of experiments, we analyzed two transgenic A. thaliana lines. The transgenic lines contained a transgene consisting of coding sequences for seryl-tRNA synthetase (SerRS) and a tag (either GFP or TAP) under regulation of a constitutive promoter. Using RT-qPCR we determined overexpression levels of SerRS transgenes. Western blot analysis and aminoacylation assays showed that the fusion SerRS proteins are active. The two transgenic lines and wild type A. thaliana seedlings were grown on medium containing different stressors (cadmium, salt, and mannitol) and the length of the roots was monitored for several days. Our results indicate that AARSs could have a role in cellular response to abiotic stress in plants.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA