Naslov
Herpes simplex virus 1 miRNA sequence variations in latently infected human trigeminal ganglia

Autori
Cokarić Brdovčak, Maja ; Zubković, Andreja ; Ferenčić, Antun ; Šoša, Ivan ; Stemberga, Valter ; Cuculić, Dražen ; Rokić, Filip ; Vugrek, Oliver ; Hackenberg, Michael ; Jurak, Igor

Izvornik
Virus research (0168-1702) 256 (2018); 90-95

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HSV-1 ; miRNA ; latency ; human trigeminal ganglia ; sequence variation

Sažetak
Human herpes simplex virus 1 (HSV-1) expresses numerous miRNAs, the function of which is not well understood. Several qualitative and quantitative analyses of HSV-1 miRNAs have been performed on infected cells in culture and animal models, however, there is very limited knowledge of their expression in human samples. We sequenced small-RNA libraries of RNA derived from human trigeminal ganglia latently infected with HSV-1 and Varicella zoster virus (VZV) and detected only a small subset of HSV-1 miRNA. The most abundantly expressed miRNAs are miR- H2, miRNA that regulates the expression of immediate early gene ICP0, and miR-H3 and -H4, both miRNAs expressed antisense to the transcript encoding the major neurovirulence factor ICP34.5. The sequence of many HSV-1 miRNAs detected in human samples was different from the sequences deposited in miRBase, which might significantly affect targeted functional analyses.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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