Naslov
Chronic sucrose intake increases expression of SREBP-1c and inflammatory response genes in rat kidney without significant changes in long chain polyunsaturated fatty acids content

Autori
Mašek, Tomislav ; Filipović, Natalija ; Galan, Asier ; Starčević, Kristina

Izvornik
Veterinarski arhiv (0372-5480) 88 (2018), 4; 497-510

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chronic inflammation, kidney, lipogenesis, glomerulosclerosis

Sažetak
We studied the influence of long-term treatment with sucrose in drinking water on the kidney histology, inflammation gene expression and lipogenesis. Male Wistar rats were supplemented with 30% sucrose (w/v) in water (MetSyn group) or with plain water (Control group). Kidney histology showed that the sucrose treated rats had increased renal lipid content and glomerulosclerosis. TGF-ß1 and TNF-α gene expression was also increased in the MetSyn group indicating low-grade inflammation. Fatty acid profile was characterized by increased saturated and monounsaturated fatty acids and decrease in C18 essential fatty acids. The content of arachidonic and docosahexaenoic acids did not decrease despite the decrease in the content of their precursors. Neither the expression nor the protein quantity of delta-5 desaturase increased indicating that renal delta-5-desatuaration is not important for the preservation of the kidney arachidonic and docosahexaenoic acid content in metabolic syndrome. The expression of SREBP-1c transcriptional factor increased two-fold in the MetSyn group indicating increased renal lipogenesis in the sucrose treated rats. In conclusion, results show that chronic sucrose intake induces glomerulosclerosis, low-grade inflammation, increases saturated and monounsaturated fatty acids and decreases C18 fatty acids without further decrease in arachidonic and docosahexaenoic fatty acids content.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA