Naslov
NMR study of nano-bio interface: A case of interaction between gold nanoparticles and biothiols

Autori
Pem, Barbara ; Vrček, Valerije ; Ćurlin, Marija ; Domazet Jurašin, Darija ; Vinković Vrček, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Adriatic NMR Conference : Book of abstracts / Bregović, Nikola ; Namjesnik, Danijel ; Novak, Predrag ; Pičuljan, Katarina - Zagreb : Odsjek za kemiju, Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, 2018, 49-49

ISBN
978-953-6076-42-0

Skup
Adriatic NMR 2018

Mjesto i datum
Mali Ston, Hrvatska, 15.06.2018. - 17.06.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gold nanoparticles ; biothiols ; NMR ; cysteine ; glutathione

Sažetak
The growing interest in utilization of gold nanoparticles (AuNPs) for diagnostics and treatment of various diseases [1] generates the need to examine their interactions with biological systems. Upon contact with biological media, NPs are immediately coated with a layer of adsorbed biomolecules called “corona” [2]. However, detailed knowledge still lacks about the nature and mechanisms behind the nano-bio interactions. One of the possible binding mechanisms is through the thiol groups, considering the high affinity of sulphur for metals. Endogenous biothiols such as cysteine and glutathione are therefore useful as models for studying the mechanism of nano-bio interactions [3]. In this study, 1H NMR was employed to study the interactions of AuNPs with cysteine and glutathione. Nanoparticles were synthesized by reduction of tetrachloroauric acid with sodium borohydride in the presence of cysteine or glutathione as stabilizing agents. 1H NMR spectra were recorded immediately after mixing the reagents, and subsequently every 20 minutes until AuNPs formation finished. Process of interaction between CYS and GSH with the AuNPs surface was observed by recording chemical shifts of signals corresponding to protons close to the thiol group, along with the loss of resolution and peak broadening. Furthermore, the intermediate spectra were captured, with distinct peaks corresponding to both bound and unbound biothiols. The results suggest the thiol group of CYS and GSH is the most responsible for biothiols-nanoparticle binding. In addition, AuNPs stabilized with CYS and GSH were characterized by means of size distribution, surface charge and visualized by transmission electron microscopy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija

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