Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells

Ressler, Antonia; Ródenas-Rochina, Joaquin; Ivanković, Marica; Ivanković, Hrvoje; Rogina, Anamarija; Gallego Ferrer, Gloria

doi:10.1016/j.carbpol.2018.06.029

Pregled bibliografske jedinice broj: 943065

Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells

Ressler, Antonia; Ródenas-Rochina, Joaquin; Ivanković, Marica; Ivanković, Hrvoje; Rogina, Anamarija; Gallego Ferrer, Gloria

Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells // Carbohydrate polymers, 197 (2018), 469-477 doi:10.1016/j.carbpol.2018.06.029 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 943065 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells

Autori
Ressler, Antonia ; Ródenas-Rochina, Joaquin ; Ivanković, Marica ; Ivanković, Hrvoje ; Rogina, Anamarija ; Gallego Ferrer, Gloria

Izvornik
Carbohydrate polymers (0144-8617) 197 (2018); 469-477

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chitosan ; differentiation ; hydrogel ; hydroxyapatite ; MSCs ; osteogenesis

Sažetak
Injectable hydrogels have emerged as promising biomaterials for tissue engineering applications. The goal of this study was to evaluate the potential of a pH-responsive chitosan- hydroxyapatite hydrogel to be used as a threedimensional support for encapsulated mesenchymal stem cells (MSCs) osteogenic differentiation. In vitro enzymatic degradation of the hydrogel, during 28 days of incubation, in simulated physiological condiditons, was characterized by swelling measurements, molecular weight determination and SEM analysis of hydrogel microstructure. Osteogenic differentiation of encapsulated MSCs was confirmed by osteogenic Runx2, collagen type I and osteocalcin immunostaining and alkaline phosphatase quantification. The deposition of late osteogenic markers (calcium phosphates) detected by Alizarin red and von Kossa staining indicated an extracellular matrix mineralization.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

Napomena
Glavni autori: Ressler Antonia i Ivanković Marica

POVEZANOST RADA

Projekti:
HRZZ-IP-2014-09-3752 - Razvoj biokompatibilnih materijala na temelju hidroksiapatita za primjene u inženjerstvu koštanog tkiva (HaTEA) (Ivanković, Hrvoje, HRZZ - 2014-09) ( CroRIS)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Hrvoje Ivanković (autor)