Pregled bibliografske jedinice broj: 941418
Initial characterization of dog serum phosphoproteome
Initial characterization of dog serum phosphoproteome // Proteomics Methods Forum 2017
Oxford, Ujedinjeno Kraljevstvo, 2017. (predavanje, podatak o recenziji nije dostupan, neobjavljeni rad, znanstveni)
CROSBI ID: 941418 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Initial characterization of dog serum phosphoproteome
Autori
Galan, Asier ; Horvatić, Anita ; Guillemin, Nicolas ; Kuleš, Josipa ; Bilić Petra ; Mrljak, Vladimir
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni
Skup
Proteomics Methods Forum 2017
Mjesto i datum
Oxford, Ujedinjeno Kraljevstvo, 22.06.2017. - 23.06.2017
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Podatak o recenziji nije dostupan
Ključne riječi
dog, serum, phosphoproteome, babesiosis
Sažetak
Phosphorylation is the most commonly studied protein post-translational modification (PTM) in biological systems due to its importance in controlling division, survival, cell growth etc. Nevertheless, there is little information on general phosphoproteomic characterization and description of the content of circulating phosphoproteins. We have performed a gel-free analysis of dog serum samples using LC-MS/MS supported by phosphopeptide enrichment. We compared serum from healthy dogs and dogs affected by B. canis-caused babesiosis. After digestion, phosphopeptides present in samples were enriched using TiO2 magnetic beads. Our approach was both qualitative and quantitative using TMT-labeled serum pool samples in combination with LC-MS/MS.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Veterinarska medicina
POVEZANOST RADA
Projekti:
HRZZ-IP-2013-11-4135 - Proteomski pristup otkrivanju biomarkera ranog oštećenja bubrega i srca u pasa (BioDog) (Mrljak, Vladimir, HRZZ - 2013-11) ( CroRIS)
Ustanove:
Veterinarski fakultet, Zagreb
