Naslov
Atopic dermatitis: immunophenotyping of inflammatory cells in skin lesions

Autori
Lugović, Liborija ; Lipozenčić, Jasna ; Jakić-Razumović, Jasminka

Izvornik
International Journal of Dermatology (0011-9059) 40 (2001); 489-494

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prikaz, znanstveni

Ključne riječi
Immunophenotyping; atopic dermatitis; inflammatory cells

Sažetak
Background. The ever increasing incidence of atopic dermatitis (AD) has stimulated many authors to use various diagnostic procedures to obtain new data that would help elucidate the pathogenesis of the disease. Methods. Immunohistochemical analysis was performed in a group of AD patients and compared with a control group of healthy subjects. The method of avidin-biotin immunoperoxidase on paraffin 4-mm skin sections, and semiquantitative counting of cells labeled with anti-CD3, anti-CD8, anti-CD20, anti-HLA-DR and anti-IgE primary antibodies were used. The aims of this pilot study were to perform cell immunophenotyping and to analyze the presence of inflammatory cell-surface markers in the biopsies of skin lesions from 15 AD patients and 5 healthy subjects. Results. Results of AD skin analysis showed a greater infiltration of CD3+ lymphocytes, especially of CD4+ subtype, compared with CD8+ lymphocytes. AD skin biopsy specimens also showed a higher intraepidermal HLA-DR+ Langerhans' cell count, presence of HLA-DR on lymphocytes in the dermis, and higher intraepidermal expression of IgE+ cells as compared with healthy controls. Conclusion. A statistically significant difference (p<0.05) was found between the two groups for intradermal and intraepidermal CD3, CD4 and HLA-DR, intradermal CD8, and intraepidermal IgE+ cells. Immunophenotyping was found to be a useful diagnostic method in AD patients.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA