Naslov
Chlamydia psittaci in free-range chicken flocks in Croatia

Autori
Quilicot, Ana M.M. ; Horvatek Tomić, Danijela ; Gottstein, Željko ; Prukner-Radovčić, Estella

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, ostalo, znanstveni

Skup
16. Deutscher Chlamydien Workshop

Mjesto i datum
Düsseldorf, Njemačka, 21.03.2018. - 23.03.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
C. psittaci, free-range chicken flocks, Croatia

Sažetak
The presence of avian chlamydia in chicken flocks affects the production and health performance of affected chickens, but it also has significant importance as zoonotic agent. Recently, in addition to C. psittaci, C. gallinacea and C. avium were also identified as avian pathogens. Although C. gallinacea is more prevalent in chicken flocks than C. psittaci, due to the pathogenicity and zoonotic potential of the latter, this study evaluated the presence of C. psittaci in free-range chicken flocks raised in Croatia. DNA was extracted from triple swabs (conjunctival, pharyngeal and cloacal), litter-feces and/or water samples collected from 54 free-range chicken flocks. Chlamydia psittaci was detected by qPCR assay by targeting the incA gene. A sample was considered positive when quantification cycle (Cq) value was < 38. Only four flocks were found to be positive for C. psittaci. Chlamydia psittaci - positive samples were further sequenced for the identification of the genotypes. Phylogenetic analysis identified genotype B and revealed the similarities and relatedness of Croatian samples and other available isolates from other countries. The presence of C. psittaci in free-range chicken flocks indicates the importance of implementing effective biosecurity and hygienic measures, as these flocks are mostly not housed in confinement. Controlling the spread of C. psittaci both to susceptible avian species and to humans is important considering its effect on poultry and its zoonotic potential.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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