Naslov
HCOMET Network: results of the first statistical analysis and new findings in the buccal micronucleus assay, as biomarkers in human biomonitoring and early disease detection

Autori
Milić, Mirta ; Bonassi, Stefano ; Rojas, Emilio ; Bolognesi, Claudia ; Consortium, hCOMET

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of 10th Congress of Toxicology in Developing Countries (IUTOX CTDC10) and 12th Congress of the Serbian Society of Toxicology (12th SCT) / Matović, vesna - Beograd, 2018, 42-42

ISBN
978-86-917867-1-7

Skup
10th Congress of Toxicology in Developing Countries (CTDC 10) ; 12th Serbian Congress of Toxicology

Mjesto i datum
Beograd, Srbija, 18.04.2018. - 21.04.2018

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
comet assay ; human biomonitoring ; buccal micronucleus assay ; human biomarkers ; early disease detection

Sažetak
The alkaline comet assay is a test for measuring single strand DNA breaks in eukaryotic cells. Method exists for more than 30 years and is used in genotoxic research. Since 2014 (revised 2016) it has become the official method for assessing genomic damage in vivo after exposure to both physical and/or chemical agents (Organization for Economic Co-operation and Development- OECD Test No. 489). With its additional variants it is also used in the biomonitoring of exposed populations, but also as an auxiliary method for disease diagnosis and in therapies for patients’ health improvement, but for such an official application, the method should be improved and validated. That task has been addressed by the newly formed group hCOMET that has gathered results on human biomonitoring studies from 41 laboratories, including 90 methods, 108 studies and more than 19 000 individuals. We will discuss the efforts and the results of the group, and will also talk about a relatively new method of micronucleus buccal test (buccal mucosa) that has a potential to replace or to be added value to the classical in vitro micronucleus method on mammalian cells. Much shorter assay than the classical micronucleus method in vitro, the assay has already shown possible application in the study of exposure to asbestos and the occurrence of Alzheimer’s disease. We will discuss the efforts already done in this area and further steps.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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