Naslov
Human intervention trial with strawberry tree (Arbutus unedo L.) honey: Impact on DNA stability and haematological parameters

Autori
Jurič, Andreja ; Mladinić, Marin ; Želježić, Davor ; Pezer, Marija ; Turkalj, Mirjana ; Jurica, Karlo ; Kopjar, Nevenka ; Brčić Karačonji, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Congress of Toxicology in Developing Countries (CTDC10) & 12th Congress of the Serbian Society of Toxicology (12th SCT): Book of Abstracts / Matović, Vesna - Beograd : Serbian Society of Toxicology, 2018, 112-112

ISBN
978-86-917867-1-7

Skup
10th Congress of Toxicology in Developing Countries (CTDC 10) ; 12th Serbian Congress of Toxicology

Mjesto i datum
Beograd, Srbija, 18.04.2018. - 21.04.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
alkaline comet assay ; challenge test ; supplementation

Sažetak
Due to its high phenol content, strawberry tree honey possesses many beneficial health effects. The aim of this study was to evaluate potential DNA and cytoprotective effects of A. unedo honey after shortterm supplementation. Participants (six healthy men, aged 26-38) consumed 70 g of honey (from Pelješac peninsula, Croatia) dissolved in 200 mL of water daily for 14 days. Their blood samples were collected before honey supplementation and one day after the last consumption (day 15). We monitored changes in leukocyte DNA integrity and haematological parameters. To establish how the supplementation modified the response of DNA to external stimuli with a genotoxic chemical, we performed a challenge test with hydrogen peroxide (H2O2) by measuring the levels of spontaneous and H2O2- induced DNA damage using the alkaline comet assay. Results showed significant lowering of comet tail intensity compared to the baseline value (0.21% vs. 0.88% DNA in comet tail). We found significant DNA-protective effects in leukocytes of almost all participants after an ex vivo challenge with H2O2. Comet tail intensity recorded after the H2O2 challenge was significantly lower after honey supplementation (2.42% DNA at day 0 vs. 0.38% DNA at day 15). Additionally, a significant increase in neutrophil count was observed after honey supplementation. Although limited by a small number of participants, the results of this intervention trial point to strawberry tree honey having promising DNA- and cytoprotective effects, which should be further clarified in forthcoming studies using other complementary methods on a much larger population exposed for longer periods.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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