Naslov
Profiling and genetic control of the murine immunoglobulin G glycome

Autori
Krištić, Jasminka ; Zaytseva, Olga O. ; Ram, Ramesh ; Nguyen, Quang ; Novokmet, Mislav ; Vučković, Frano ; Vilaj, Marija ; Trbojević-Akmačić, Irena ; Pezer, Marija ; Davern, Kathleen M. ; Morahan, Grant ; Lauc, Gordan

Izvornik
Nature chemical biology (1552-4450) 14 (2018), 5; 516-524

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
IgG glycosylation

Sažetak
Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter- individual variability are not well defined. The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse. By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences ; (ii) five genetic loci were found to be associated with murine IgG glycosylation ; (iii) variants outside traditional glycosylation site motifs affected glycome variation ; (iv) bisecting N- acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs ; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA