Naslov
MOLECULAR EPIDEMIOLOGY OF BRUCELLA MELITENSIS STRAINS CAUSING OUTBREAKS IN CROATIA AND BOSNIA AND HERZEGOVINA

Autori
Duvnjak, Sanja ; Račić Ivana ; Špičić, Silvio ; Zdelar-Tuk, Maja ; Reil, Irena ; Cvetnić, Željko

Izvornik
Acta veterinaria Hungarica (0236-6290) 66 (2018), 2; 177-188

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Brucella melitensis, MLVA, MLST, Croatia, infectious disease

Sažetak
The most recent data on brucellosis incidence in South East (SE) Europe prove the persistence of this zoonosis in the area, regardless constant efforts to control one of the most dangerous zoonosis. Forty three Brucella melitensis strains were gathered originating from cattle, sheep, goats and humans from Croatia and Bosnia and Herzegovina between 2009 and 2015, identified and genotyped in order to access the epidemiological background of the strains. Standard biotyping methods and Bruce-ladder were used to identify the strains. Genotyping was done using MLVA on 16 and MLST on 9 loci. Results were compared to each other and to internationally available data. Twenty five novel genotypes and two sequence types were identified. All tested strains, apart from vaccine and referent strains showed very close phylogenetic and geographic relationship. Genotyping results point to endemicity of brucellosis in this region. MLST showed no variation affirming the stability of housekeeping genes. Results confirm already established routes of disease spread in this area showing that more detailed and vigorous control of this zoonosis is necessary.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA