Naslov
The Effect of Intra-articular Injection of Autologous Microfragmented Fat Tissue on Proteoglycan Synthesis in Patients with Knee Osteoarthritis

Autori
Hudetz, Damir ; Borić, Igor ; Rod, Eduard ; Jeleč, Željko ; Radić, Andrej ; Vrdoljak, Tomislav ; Skelin, Andrea ; Lauc, Gordan ; Trbojević Akmačić, Irena ; Plećko, Mihovil ; Polašek, Ozren ; Primorac, Dragan

Izvornik
Genes (2073-4425) 8 (2017), 217; 1-17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mesenchymal stem cell ; knee osteoarthritis ; adipose tissue ; regenerative medicine ; dGEMRIC ; glycosaminoglycans ; cartilage

Sažetak
Osteoarthritis (OA) is one of the leading musculoskeletal disorders in the adult population. It is associated with cartilage damage triggered by the deterioration of the extracellular matrix tissue. The present study explores the effect of intra-articular administration of autologous microfragmented adipose tissue containing adipose- derived mesenchymal stem cells (Ad-MSCs) to host chondrocytes and cartilage proteoglycans in patients with knee OA. A prospective, non- randomized, interventional, single-center, open- label clinical trial was conducted from January 2016 to April 2017. A total of 17 patients were enrolled in the study, and 32 knees with osteoarthritis were assessed. Surgical intervention (lipoaspiration) followed by tissue processing and intra-articular injection of the final product containing a derivate of micronized fatty tissue with pericytes and mesenchymal stem cells (Ad-MSCs) into the affected knee(s) was performed in all patients. Patients were assessed for Visual Analogue Scale (VAS), dGEMRIC MRI imaging and immunoglobulin G (IgG) glycans at the baseline, 3, 6 and 12 months after the treatment. T1 relaxation time in dGEMRIC due to infiltration of the anionic, negatively charged contrast Gd- DTPA2− into the cartilage indicated that the contents of cartilage glycosaminoglycans significantly increased in specific areas of the treated knee joint. In addition, dGEMRIC MRI imaging consequently reflected subsequent changes in the mechanical axis of the lower extremities. The results of our study indicate that the use of (Ad-MSC) in patients with knee OA (measured by dGEMRIC MRI) increased glycosaminoglycans (GAGs) content in hyaline cartilage, which is in line with observed VAS and clinical results.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA