Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Banić, Martina; Uroić, Ksenija; Leboš Pavunc, Andreja; Novak, Jasna; Zorić, Katarina; Durgo, Ksenija; Petković, Hrvoje; Jamnik, Polona; Kazazić, Saša; Kazazić, Snježana; Radović, Slobodanka; Scalabrin, Simone; Hynӧnen, Ulla; Šušković, Jagoda; Kos, Blaženka

doi:10.1016/j.lwt.2018.03.054

Pregled bibliografske jedinice broj: 933748

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Banić, Martina; Uroić, Ksenija; Leboš Pavunc, Andreja; Novak, Jasna; Zorić, Katarina; Durgo, Ksenija; Petković, Hrvoje; Jamnik, Polona; Kazazić, Saša; Kazazić, Snježana et al.

Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut // LWT-Food Science and Technology, 93 (2018), 257-267 doi:10.1016/j.lwt.2018.03.054 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 933748 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

Autori
Banić, Martina ; Uroić, Ksenija ; Leboš Pavunc, Andreja ; Novak, Jasna ; Zorić, Katarina ; Durgo, Ksenija ; Petković, Hrvoje ; Jamnik, Polona ; Kazazić, Saša ; Kazazić, Snježana ; Radović, Slobodanka ; Scalabrin, Simone ; Hynӧnen, Ulla ; Šušković, Jagoda ; Kos, Blaženka

Izvornik
LWT-Food Science and Technology (0023-6438) 93 (2018); 257-267

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lactobacillus brevis, probiotics, Slps, adhesion, microencapsulation

Sažetak
S-layers represent the simplest biological membranes developed during the evolution and are one of the most abundant biopolymers on Earth. Current fundamental and applied research aim to reveal the chemical structure, morphogenesis and function of S-layer proteins (Slps). This is the first paper that describes the Slps of certain Lactobacillus brevis strain isolated from sauerkraut. The whole genome sequence (WGS) analysis of the L. brevis SF9B strain uncovered three genes encoding the putative Slps, but merely one, identified as similar to the SlpB of L. brevis ATCC 14869, was expressed. Slp-expressing SF9B cells exhibited increased survival in simulated gastrointestinal (GI) conditions and during freeze-drying. Their survival in stress conditions was additionally enhanced by microencapsulation, especially when using alginate with gelatine as a matrix. Thus prepared cells were subjected to simulated GI conditions and their mortality was only 0.28 ± 0.45 log CFU/mL. Furthermore, a correlation between the high surface hydrophobicity and the remarkable aggregative capacity of SF9B strain was established. The results indicate a prominent role of Slps in adhesion to mucin, extracellular matrix (ECM) proteins, and particularly to Caco-2 cells, where the removal of Slps utterly abolished the adhesiveness of SF9B cells for 7.78 ± 0.25 log CFU/mL.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA

Projekti:
2439-2016
HRZZ-IP-2014-09-7009 - Probiotici i strater kulture- površinski proteini i bakteriocini (PRO-S) (Kos, Blaženka, HRZZ - 2014-09) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Ksenija Durgo (autor)