Naslov
Mannosylated Liposomes with Built-in Peptidoglycan Based Immunomodulators for Subunit Vaccine Formulations

Autori
Štimac, Adela ; Bendelja, Krešo ; Dutour Sikirić, Maja ; Frkanec, Leo ; Frkanec, Ruža

Izvornik
Croatica chemica acta (0011-1643) 90 (2017), 4; 645-656

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mannosylated liposomes ; targeted delivery ; peptidoglycan monomer ; adamantyltripeptide ; adjuvants ; subunit vaccines

Sažetak
The aim of the present study is preparation of mannosylated liposomes with built-in small molecule immunopotentiators for targeted, receptors mediated, delivery of antigens. The liposomes were mannosylated in two ways, by covalent attachment of p-aminophenyl-α-D- mannopyranoside to the preformed liposomes and by incorporation of synthetic mono-, di- and tetramannosyl-lipoconjugates into the lipid bilayer of liposomes. Four different mannosylated liposome formulations with incorporated model antigen, ovalbumin (OVA), and immunomodulators, PGM and Ad2TP2, were prepared and characterized. The influence of mannosylated liposome formulations on the antigen-specific humoral immune response was investigated. It has been shown that mannosylated liposomal formulations did not enhance the humoral immune response and production of anti-OVA antibodies but they significantly affected the type of OVA specific immune reaction and directed it towards Th1 type.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA