Naslov
Hepatic lipogenesis and brain fatty acid profile in response to different dietary n6/n3 ratios and DHA/EPA supplementation in streptozotocin treated rats

Autori
Starčević, Kristina ; Filipović, Natalija ; Galan, Asier ; Micek, Vedran ; Gudan Kurilj, Andrea ; Mašek, Tomislav

Izvornik
Molecular Nutrition and Food Research (1613-4125) 62 (2018), 9; 1701007, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
fatty acids ; RAGE ; lipid oxidation ; diabetes type 1 ; delta-desaturase ; β-oxidation

Sažetak
Scope: We investigated the interaction between streptozotocin-induced diabetes and dietary n6/n3 ratio, and its influence on lipogenesis. Methods and results: The animals were treated with streptozotocin (STZ) and fed with different dietary n6/n3 ratios: 1, 7 and 60, or supplemented with DHA/EPA. Gene expression was assessed by RT-PCR and protein expression by western blotting and immunohistochemistry. Fatty acid profile was determined by GC-MS. Pancreas and liver histology were assessed by H&E staining. Streptozotocin induced characteristic changes in all STZ treated groups, including: increased blood glucose, decreased body mass, increased lipid peroxidation and CD36 expression, decreased 16:1n7 and 18:1n7, increases in 20:3n6, decreases in phospholipid content of 20:4n6, as well as decreases in the expression of SREBP1c, Δ-9-desaturase and Δ-5-desaturase. Additionally other changes occurred that were dependent on the n6/n3 ratio. Among the diabetic groups, the lower n6/n3 ratio caused higher lipid peroxidation and CD36 expression, a greater decrease in 20:4n6 and decreased Δ6-desaturase expression, while the higher n6/n3 ratio caused increased partitioning of 20:4n6 into hepatic neutral lipids, a decrease in 20:5n3 content and increased β-oxidation. Conclusion: Presented data suggest that that n6/n3 ratio could significantly influence lipogenesis, lipid peroxidation and β-oxidation in STZ- induced diabetes, which could have clinical significance.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina

POVEZANOST RADA