Naslov
A chemical and biological investigation of Lepidium latifolium L. (pepperweed)

Autori
Azra Đulović, Vedrana Čikeš-Čulić, Ana Maravić, Sabine Montaut, Patrick Rollin, Ivica Blažević

Kolaboracija
Laurentian University (Canada), Université d’Orléans et CNRS, ICOA (france)

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
25th Young Research Fellows' Meeting / - Orléans : French Medicinal Chemistry Society, 2018, 109-109

Skup
25th Young Research Fellows' Meeting

Mjesto i datum
Orléans, Francuska, 05.03.2018. - 07.03.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lepidum latifolium, glucosinolates, isothiocyanates, anticarcinogenic activity, antimicrobial activity

Sažetak
The genus Lepidium (Brassicaceae) includes about 175 different plant species strikingly characterized by glucosinolates (GLs) as molecular tags [1]. The cultivated Lepidium latifolium L. (perennial pepperweed) was investigated to decipher its GL profile using 1) an indirect method with GC-MS analysis of the GL degradation volatile products (mostly isothiocyanates and nitriles) obtained by hydrodistillation and organic solvent extraction, and 2) a direct method with LC-MS analysis of intact GLs. GC-MS analysis showed that the most abundant GLs were sinigrin and glucotropaeolin, and a minor one was glucocochlearin. LC-MS analysis confirmed the presence of the above-mentioned GLs but also detected 4-methoxyglucobrassicin. In recent years, studies have been directed towards isothiocyanates due to the broad range of their biological effects [1-3]. Taking that into account, L. latifolium leaf extract and hydrodistillate containing GL degradation products were evaluated for their anticarcinogenic activity against two human tumor cell lines - glioblastoma cell line LN-229 and bladder cancer cell line UM-UC-3. Their antimicrobial activity against pathogenic and food spoilage bacteria and fungi was also assessed. The extract and distillate exhibited very similar anticarcinogenic and antibacterial activities, but with a somewhat better effect for the extract. On the other hand, both extract and distillate were found to exert very strong antifungal activity against Candida albicans (MIC50 8 and 16 µg/mL).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA