Naslov
Protozoan and helminth parasite fauna of free- living Croatian wild wolves (Canis lupus) analyzed by scat collection

Autori
Hermosilla, Carlos ; Kleinertz, Sonja ; Silva, Liliana M.R. ; Hirzmann, Jörg ; Huber, Djuro ; Kusak, Josip ; Taubert, Anja

Izvornik
Veterinary Parasitology (0304-4017) 233 (2017); 14-19

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Canis lupus ; Angiostrongylus vasorum ; Giardia ; Cryptosporidium ; oxocara ; Sarcocystis ; Croatia
(Canis lupus Angiostrongylus vasorum Giardia Cryptosporidium Toxocara Sarcocystis Croatia)

Sažetak
The European wolf (Canis lupus) is a large carnivore species present in limited areas of Europe with sev- eral small populations still being considered as endangered. Wolves can be infected by a wide range of protozoan and metazoan parasites with some of them affecting free-living wolf health condition. On this account, an epidemiological survey was conducted to analyze the actual parasite fauna in Croatian wild wolves. In total, 400 individual faecal samples were collected during field studies on wolf ecol- ogy in the years 2002–2011. Parasite stages were identified by the sodium acetate acetic acid formalin (SAF)-technique, carbolfuchsin-stained faecal smears and Giardia/Cryptosporidium coproantigen-ELISAs. A subset of taeniid eggs-positive wolf samples was additionally analyzed by PCR and subsequent sequenc- ing to identify eggs on Echinococcus granulosus/E. multilocularis species level. In total 18 taxa of parasites were here detected. Sarcocystis spp. (19.1%) occurred most frequently in faecal samples, being followed by Capillaria spp. (16%), ancylostomatids (13.1%), Crenosoma vulpis (4.6%), Angiostrongylus vasorum (3.1%), Toxocara canis (2.8%), Hammondia/Neospora spp. (2.6 %), Cystoisospora ohioensis (2.1%), Giardia spp. (2.1%), Cystoisospora canis (1.8%), Cryptosporidium spp. (1.8%), Trichuris vulpis (1.5%), Taenia spp. (1.5%), Diphyl- lobothrium latum (1.5%), Strongyloides spp. (0.5%), Opisthorchis felineus (0.5%), Toxascaris leonina (0.3%), Mesocestoides litteratus (0.3%) and Alaria alata (0.3%). Some of the here identified parasites represent relevant pathogens for wolves, circulating between these carnivorous definitive hosts and a variety of mammalian intermediate hosts, e. g. Taenia spp. and Sarcocystis spp., while others are considered exclusively pathogenic for canids (e.g. A. vasorum, C. vulpis, T. vulpis, Cystoisospora spp.). This study pro- vides first records on the occurrence of the two relevant anthropozoonotic parasites, Giardia spp. and Cryptosporidium spp., in wild wolves from Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

POVEZANOST RADA